| 血糖波动对糖尿病大鼠胰岛素释放和胰岛素敏感性的影响 |
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| 引用本文： | 蔡 燕,刘翠萍,茅晓东,陈国芳,徐 瑜,刘 超.血糖波动对糖尿病大鼠胰岛素释放和胰岛素敏感性的影响[J].南京医科大学学报,2009,29(1):60-64. |
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| 作者姓名： | 蔡 燕 刘翠萍 茅晓东 陈国芳 徐 瑜 刘 超 |
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| 作者单位： | 南京医科大学第一附属医院内分泌科,江苏,南京,210029
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| 基金项目： | 江苏医学领军人才基金 |
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| 摘 要： | 目的:观察血糖波动对糖尿病大鼠胰岛素释放和胰岛素敏感性的影响.方法:GK大鼠22只,随机分为糖尿病高血糖组、糖尿病血糖波动组,Wistar大鼠11只作为正常对照组,进行葡萄糖耐量实验(IPGTT)及在体胰岛分离后体外葡萄糖刺激下胰岛素释放实验(GSIS),并检测胰岛素相关基因的表达,以了解β细胞的胰岛素释放功能,进行胰岛素耐量试验以检测胰岛素敏感性的情况.结果:实验6周后血糖波动组和持续高血糖组体重无统计学差异.血糖波动组空腹血糖,糖负荷后15、30、60、120 min血糖,糖曲线下面积(AUCG)均高于持续高血糖组(P均<0.05).在体胰岛分离后体外葡萄糖刺激下胰岛素释放指数明显低于持续高血糖组(P均<0.05).血糖波动组胰岛素基因ins-1、ins-2和pdx-1表达及胰岛素敏感性较持续高血糖组显著降低(P均<0.05).结论:血糖波动使GK大鼠胰岛素释放功能降低并且胰岛素相关基因表达减少可能是胰岛素释放减少的原因之一,血糖波动使胰岛素敏感性降低.
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| 关 键 词： | 血糖波动 2型糖尿病 胰岛素 胰岛素敏感性 |
| 收稿时间： | 2008/8/10 0:00:00 |
Effects of intermittent high glucose on insulin release and insulin sensitivity in diabetic rats |
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| CAI Yan,LIU Cui-ping,MAO Xiao-dong,CHEN Guo-fang,XU Yu and LIU Chao.Effects of intermittent high glucose on insulin release and insulin sensitivity in diabetic rats[J].Acta Universitatis Medicinalis Nanjing,2009,29(1):60-64. |
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| Authors: | CAI Yan LIU Cui-ping MAO Xiao-dong CHEN Guo-fang XU Yu and LIU Chao |
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| Institution: | Department of Endocrinology;the First Affiliated Hospital of NJMU;Nanjing 210029;China |
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| Abstract: | Objective:To compare the effects of intermittent high blood glucose on insulin release and insulin sensitivity in GK rats. Methods:Twenty-two male GK rats were randomly divided into 2 groups,which were consistent high blood glucose group(HG) and intermittent high blood glucose group (FG). Eleven male wistar rats were used as normal glucose controls (NG). The fluctuant high blood glucose animal model was induced by intrapentoneal injection of insulin and glucose at different time for 6 weeks. After the intro... |
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| Keywords: | intermittent high blood glucose diabetes insulin insulin sensitivity |
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