核黄素对烧伤后早期损害的防治作用

采用大鼠３０％Ⅲ度烧伤模型，伤后立即予以腹腔注射核黄素，通过尿量、动物死亡率统计、血浆丙二醛、血清皮质醇、尿儿茶酚胺以及血容量和不同组织器官微血管通透性的观察测定，并以单纯烧伤和正常动物作对照，结果显示：①核黄素治疗后烧伤动物休克期尿量始终高于未治疗的单纯烧伤组；②治疗后的动物血浆丙二醛含量明显下降；③治疗组血清皮质醇水平和尿肾上腺素含量相对稳定；④血浆和组织伊文思蓝－蛋白质含量的测定结果，治疗组血浆、肝、肾以及痂下组织的伊文思蓝－蛋白质浓度均低于单纯烧伤组；⑤治疗组伤后２４ｈ存活率显著高于单纯烧伤组。提示烧伤早期核黄素的运用能维持相对较充足的血容量；同时减轻机体伤后剧烈的应激反应和机体脂质过氧化损害；在整体达到提高烧伤早期动物存活率的效果

Study of effects of riboflavin on systemic damages in early stage postburn in rats

After the rats were inflicted with 30% TBSA full thickness burns, they were intraperitoneally injected with riboflavin and then blood and urine volume, level of urine riboflavin, plasma malondialdehyde, serum cortisol and urine catecholamines, and microvascular permeability of various tissues and organs were determined. Those of the normal rats and burnt rats without riboflavin treatment were determined likewise to serve as the negative and positive controls. The findings were as follows: (1) The urine volume was higher in the riboflavin treated rats than in those without treatment in the shock stage ( P <0.01). (2) The plasma malondialdehyde level was significantly lower in the riboflavin treated rats. (3) The level of plasma cortisol and urine epinephrine were maintained relatively stable in the treated rats. (4) The Evans blue protein content in the plasma, liver, kidneys and subeschar tissues was lower in the treated rats than in those without treatment. (5) The survival rate of the burnt rats in the 24th hour postburn was significantly higher in the treated rats than in those without. These findings suggest that the administration of riboflavin in the early stage postburn can maintain a reasonably sufficient blood volume and prevent drastic stress reaction and lipid peroxidation from occurring. Consequently, the survival rate of the rats is improved.