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In vitro assembly of poliovirus-related particles
Authors:B A Phillips  D F Summers  J V Maizel
Affiliation:5. Department of Cell Biology, Albert Einstein College of Medicine, New York, New York 10461 USA;6. Department of Microbiology and Immunology, Albert Einstein College of Medicine, New York, New York 10461 USA;1. Département de radiothérapie, centre de lutte contre le cancer Paul-Strauss, 3, rue de la Porte-de-l’Hôpital, 67065 Strasbourg, France;2. Département de radiothérapie, institut de cancérologie de l’Ouest René-Gauducheau, boulevard Jacques-Monod, 44805 Saint-Herblain cedex, France;3. Département de radiothérapie, centre Jean-Perrin, 58, rue Montalembert, 63000 Clermont-Ferrand, France;4. Département de radiothérapie, institut du cancer Courlancy, 38, rue de Courlancy, 51100 Reims, France;5. Laboratoire EA 3430, Fédération de médecine translationnelle de Strasbourg (FMTS), université de Strasbourg, 67000 Strasbourg, France;1. Department of Neurology, University Hospital Centre Zagreb, School of Medicine, University of Zagreb, Zagreb, Croatia;2. School of Medicine, University of Zagreb, Zagreb, Croatia;3. Department of Physiotherapy, University of Applied Health Sciences, Zagreb, Croatia;4. Danish Headache Centre, Department of Neurology, Rigshospitalet Glostrup, University of Copenhagen, Copenhagen, Denmark;5. Department of Food Quality Control, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Abstract:A viral fraction has been isolated from poliovirus-infected HeLa cells which sediments at 10 S and which contains virus-specific polypeptides closely resembling those of 73 S empty capsids. In the presence of infected cell extracts, the 10 S material is assembled to a 73 S product indistinguishable from natural empty capsids by sedimentation behavior or by polypeptide analysis with gel electrophoresis. Kinetic experiments suggest that the 10 S and 73 S components are sequential precursors of the complete virion.
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