Comparison of four non-radioactive and 35S-based methods for the detection of human papillomavirus DNA by in situ hybridization. |
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Authors: | C Clavel I Binninger M C Boutterin M Polette P Birembaut |
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Affiliation: | Laboratoire Pol Bouin, INSERM U 314, H?pital Maison Blanche, Reims, France. |
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Abstract: | Human papillomavirus DNA was detected in 40 condylomatous lesions of various sites (vulva, cervix, larynx, penis and anus) by in situ hybridization using 35S-labelled probes and four non-radioactive probes to compare the various sensitivities of these techniques on the same material (formalin-fixed and paraffin-embedded sections). Radioactive probes yielded 28 positive results out of 40 (70%). Sulphonated probes (HybriCyte kit) also gave 28 positive results with a fine pattern of hybridization grains and equal sensitivity to 35S-labelled probes. Biotinylated and digoxigenin-labelled probes gave analogous results (25 positive reactions with the PathoGene kit, 26 with the Viratype kit, and 25 with digoxigenin-labelled probes) but are slightly less sensitive than radiolabelled and sulphonated probes especially when the signal is weak. |
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