重组白介素-1受体拮抗剂对实验性肝损伤保护作用的初步研究

白介素１（ＩＬ１） 是重要的炎症因子之一， 在肝损伤中起着重要的作用。为研究重组ＩＬ１ 受体拮抗剂ｒＩＬ１ｒａ） 对四氯化碳（ＣＣＩ４） 诱导的肝损伤的保护作用，将２４ 只Ｗｉｓｔａｒ 大鼠随机分为５ 组：即正常对照组（ｎ ＝ ５） ，造模组（ｎ ＝ ５） ，ｒＩＬ１ｒａ 高剂量（０ ．５ ｍ ｇ／１００ ｇ） 组（ｎ ＝ ５） ，中剂量（０ ．２ ｍｇ／１００ ｇ） 组（ｎ ＝ ５） 和低剂量（ｏ ．１ｍ ｇ／１００ ｇ） 组（ｎ ＝ ４） 。检测处理６ 周末血清肝酶学（ＡＬＴ，ＡＳＴ） 和肝纤维化指标：Ⅲ型前胶原（Ｐｃ Ⅲ） 、透明质酸（ ＨＡ） 和层粘蛋白（ＬＮ） 。结果：与造模组相比较，ｒＩＬ１ｒａ 各剂量组血清ＡＬＴ 和ＡＳＴ 的水平下降（ Ｐ ＜ ０ ．００１ 和Ｐ ＜ ０ ．０１） ，但组间无差异；而对于肝纤维化指标，虽然数值有下降趋势，但只有高剂量才具有统计学意义（ Ｐ ＜ ０ ．０５） 。表明ｒＩＬ１ｒａ（０ ．５ｍ ｇ／１００ ｇ） 可阻断肝细胞的急、慢性损伤，抑制肝纤维化的形成，从而可间接地反映ＩＬ１ 参与了ＣＣｌ４ 导致肝损伤的发病过程。

The role of recombinant interleukin 1 receptor antagonist(rIL 1ra) in protecting liver from injury induced by carbon tetrachloride in Wastar rats

To examine the effect of IL 1ra on blocking the development of experimental hepatic injury induced by carbon tetracholride(CCl4), Wistar rats were divided into five groups, namely, control (n=5), CCl4(n=5), CCl4 rIL 1ra [riL 1ra doses: low: 0.1 mg/100 g/d(n=4), median:0.2 mg/100 g/d(n=5) and high: 0.5 mg/100 g/d(n=5)], respectively. For each group, the serum levels of the ALT, AST, procollagen III (PcIII), hylauronic acid(HA) and laminin (LN) were measured. In group treated with CCl4 rIL 1ra, we observed a decreased serum levels of ALT, AST, Pc III, HA and LN as compared with those treated with CCl4 alone and the decline degrees were dose dependent with rIL 1ra in the serum levels of Pc III, HA, LN while the decline level of ALT, AST in serum did not show the relationship mentioned above. The significant difference in the serum level of ALT, AST (P < 0.001 and P < 0.01) was found in all doses of IL 1ra. but the significant difference in the serum level of PcIII, HA, LN was only found in high dose of rIL 1ra. These findings suggested that administration of rIL 1ra may be effective in preventing the developing of experimental hepatic injury and hepatic fibrosis. It suggests that IL 1 is involved in the developing of hepatic damage induced by CCl4.