| Caspase-1及其下游炎症因子在大鼠脊髓损伤中的表达及意义 |
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| 引用本文： | 锁志刚,丁惠强,飞翔,樊涛. Caspase-1及其下游炎症因子在大鼠脊髓损伤中的表达及意义[J]. 宁夏医科大学学报, 2011, 0(1): 48-52,F0004 |
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| 作者姓名： | 锁志刚 丁惠强 飞翔 樊涛 |
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| 作者单位： | 宁夏医科大学;宁夏医科大学附属医院骨科; |
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| 摘 要： | 目的探讨天冬氨酸特异性半胱氨酸蛋白酶-1（Caspase-1）及其下游炎症因子自介素-1beta（IL-1β）和白介素-18（IL-18）在大鼠脊髓损伤（spinal cord injury，SCI）中的表达及意义。方法将成年雄性Sprague—Dawley（SD）大鼠84只随机分为对照组和脊髓损伤组，每组42只。脊髓损伤组应用改良的Allen’S重物打击法建立大鼠脊髓损伤模型，对照组只做全椎板切除。以损伤部位为中心取材，HE染色观察脊髓组织病理变化，免疫组化染色检测Caspase-1、IL-1β及IL-18的表达情况。结果对照组大鼠脊髓组织中Caspase-1及其下游炎症凶子阳性细胞有少量表达，而脊髓损伤组Caspase—1及其下游炎症因子的阳性表达较对照组显著增高，Caspase-1及IL-18存脊髓损伤后3d表达达到峰值，而IL-1β于损伤后6h表达达到高峰。结论Caspase—1及其下游炎症因子可能参与了SCI后的继发性损伤，并且可能是继发性脊髓损伤的主要损伤因素之一。
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| 关 键 词： | 脊髓损伤 半胱氨酸天冬氨酸酶-1 白细胞介素-1β 白细胞介素-18 大鼠 |
Expression and Significance of Caspase-1 and Inflammatory Factor Activated by Caspase-1 in Rats with Spinal Cord Injury |
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| SUO Zhi-gang,DING Hui-qiang,FEI Xiang,FAN Tao. Expression and Significance of Caspase-1 and Inflammatory Factor Activated by Caspase-1 in Rats with Spinal Cord Injury[J]. Journal of Ningxia Medical College, 2011, 0(1): 48-52,F0004 |
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| Authors: | SUO Zhi-gang DING Hui-qiang FEI Xiang FAN Tao |
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| Affiliation: | SUO Zhi-gang1,DING Hui-qiang2,FEI Xiang2,FAN Tao2(1.Ningxia Med.Univ.,Yinchuan 750004,2.the Affiliated Hospital of Ningxia Med.Univ.Yinchuan 750004) |
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| Abstract: | Objective To investigate the expression and significance of Caspase-1 and inflammatory factor activated by Caspase-1 in rats with spinal cord injury.Methods 84 adult Sprague-dawley male rats were randomly divided into two groups:The control group(group A)and spinal cord injury group(group B),each group consisted of 42 rats.Models of SCI established by modified Allen's method in rats of Group B,group A underwent a laminectomy without injury.The lesion areas were dissected for morphological studies by hematox... |
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| Keywords: | spinal cord injury Caspase-1 interleukin-1beta interleukin-18 rat |
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