| 缺血预处理大鼠心肌组织BDNF表达及其意义 |
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| 引用本文： | 高致炳,徐清斌,徐文中,王云.缺血预处理大鼠心肌组织BDNF表达及其意义[J].宁夏医科大学学报,2011(5):414-417. |
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| 作者姓名： | 高致炳 徐清斌 徐文中 王云 |
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| 作者单位： | 宁夏医科大学;宁夏医科大学总医院心脏中心内科二病区;宁夏医科大学总医院心脏中心外科病区; |
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| 基金项目： | 宁夏回族自治区科学基金资助(H0212) |
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| 摘 要： | 目的观察缺血预处理大鼠心肌中脑源性神经生长因子（BDNF）的mRNA及蛋白表达情况，并探讨其意义。方法54只雄性SD大鼠随机分为空白对照组（Con组）、缺血再灌注组（VR组）和缺血预处理组（IPC组），VR组及IPC组模型建立后，再分别分为再灌注后1、3、6h及12h各时间点亚组。RT-PCR检测心肌组织BDNFmRNA表达，Western-blot法检N,5肌组织BDNF蛋白表达。结果VR组及IPC组各时间点大鼠心肌组织中BDNFmRNA及蛋白的表达与Con组相比，差异均有统计学意义（P〈0．05）；而L／R组与IPC组再灌注后同时间点比较大鼠心肌组织中BDNFmRNA及蛋白表达的差异也均有统计学意义（P〈0．05）。结论缺血预处理可以上调BDNF的mRNA及蛋白表达，推测BDNF在缺血预处理后对缺血再灌注大鼠心肌具有保护作用。
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| 关 键 词： | 脑源性神经生长因子 缺血再灌注 缺血预处理 |
BDNF Expression in Myocardial Ischemic Preconditioning and Its Significance |
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| Gao Zhi-bing,XU Qing-bing,XU Wen-zhong,WANG Yun.BDNF Expression in Myocardial Ischemic Preconditioning and Its Significance[J].Journal of Ningxia Medical College,2011(5):414-417. |
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| Authors: | Gao Zhi-bing XU Qing-bing XU Wen-zhong WANG Yun |
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| Institution: | Gao Zhi-bing,XU Qing-bing,XU Wen-zhong,WANG Yun(Ningxia Medical University,Yinchuan 750004) |
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| Abstract: | Objective To observe BDNF mRNA and protein expression in the myocardial ischemic preconditioning and its significance.Methods 54 male SD rats were randomly divided into control group(Con group),ischemia-reperfusion group(I/R group) and ischemic preconditioning group(IPC group).After establishment of I/R group and IPC group model,all rats were divided into 1h,3h,6h and 12h group after reperfusion.BDNF mRNA were detected by RT-PCR and the BDNF protein were detected by Western-blo.Results Compared with the Con... |
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| Keywords: | brain derived neurotrophic factor ischemia-reperfusion ischemic preconditioning |
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