| Cdk1在HepG2细胞的表达及活性对肝癌细胞凋亡的影响 |
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| 引用本文： | 李瑞,郑亚莉,周学兵,王炜. Cdk1在HepG2细胞的表达及活性对肝癌细胞凋亡的影响[J]. 宁夏医科大学学报, 2011, 0(6): 521-524,F0004 |
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| 作者姓名： | 李瑞 郑亚莉 周学兵 王炜 |
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| 作者单位： | 宁夏医科大学;宁夏回族自治区第一人民医院; |
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| 基金项目： | 宁夏回族自治区自然科学基金(NZ10162) |
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| 摘 要： | 目的观察Cdk1蛋白表达及其活性对肝癌细胞凋亡的影响。方法克隆Cdk1基因质粒和Cdk1siRNA,并将其转染到肝癌HepG2细胞中,另外应用roscovintine（Cdks的抑制剂）刺激细胞,然后给予紫外线（UV）照射。用Western Blot检测转染的Cdk1蛋白的表达,用核素标记测定其活性;用HO 33342（Hoechst 33342）的免疫荧光染色细胞核判断细胞凋亡的情况。分析Cdk1蛋白表达及活性对肝癌细胞凋亡的影响。结果 Cdk1可以在HepG2良好地表达;Cdk1siRNA可以有效地沉默Cdk1的表达;roscovintine可抑制Cdk1的活性;细胞凋亡率在roscovintine刺激细胞时最高比较Cdk1转染细胞组和Cdk1siRNA转染细胞组（P〈0.01）,在Cdk1转染细胞高于Cdk1siRNA转染细胞组（P〈0.01）和空载体转染细胞组（P〈0.05）,在Cdk1siRNA转染细胞最低。结论过度表达Cdk1可以增加肝癌细胞死亡,抑制肝癌的增殖。抑制Cdk1活性可更有效增加肝癌细胞死亡,抑制肝癌的增殖,可望成为研究和探讨肝癌靶点之一。
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| 关 键 词： | Cdk1 siRNA 细胞凋亡 激酶活性 |
Expression and Activity of CDK1 on Cell Apoptosis in Hepatoma HepG2 |
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| LI Rui,ZHENG Ya-Li,ZHOU Xue-Bin,WANG Wei. Expression and Activity of CDK1 on Cell Apoptosis in Hepatoma HepG2[J]. Journal of Ningxia Medical College, 2011, 0(6): 521-524,F0004 |
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| Authors: | LI Rui ZHENG Ya-Li ZHOU Xue-Bin WANG Wei |
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| Affiliation: | LI Rui1,ZHENG Ya-Li2,ZHOU Xue-Bin1,WANG Wei2(1.Ningxia Medical University,Yinchuan 750004,2.Ningxia First People's Hospital,Yinchuan 750021) |
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| Abstract: | Objective To observe expression and activity of Cdk1 on the cell apoptosis in HepG2 cells.Methods The Cdk1 plasmid and Cdk1siRNA were constructed and transfected into hepatoma HepG2 cells.At the same time,the cells were treated by roscivintie(a Cdk1 inhibitor).After irradiation with UV light,Cdk1 protein expression was detected by Western Blot assay;The kinase activity was analysised by gama-32P labeled kinase assay;and cell apoptosis was detected by using HO 33342(Hoechst 33342) staining.Results Cdk1 plasm... |
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| Keywords: | Cdk1 siRNA apoptosis kinase activity |
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