蛛网膜下腔出血大鼠大脑中动脉单胺能神经的变化

目的：探讨交感神经与蛛网膜下腔出血（subarachnoid hemorrhage,SAH)脑血管痉挛的发生和发展关系，为深入阐明SA发病机理提供形态学资料。方法：Wistar大鼠20只，15只为实验组。用自体动脉血注入大鼠蛛网膜下池建立SAH模型，另5只为对照组，应用免疫组织化学ABC法，对对照组和大鼠SAH后第2、7、14天大脑中动脉单胺能神经分布进行观察，结果：对照组及SAH大鼠的大脑中动脉均可见棕褐色细线、弯曲状的免疫反应阳性纤维，SAH鼠与对照鼠比较，纤维密度明显减少，经统计学检验，P＜0．05，表明SAH鼠与对照鼠的大脑中动脉的单胺能神经纤维的密度有明显差异。结论：交感神经可能在SAH鼠脑血管活动和对脑痉挛的发生和发展起重要作用。

Changes of The Monoaminergic Nerves on The Middle Cerebral Arteries in Subarachnoid Hemorrhage Rats

Objective: To explore the relationship between sympathetic nerve and cerebral vasospasm. Methods: 20 Wistar rats were divided into experiment group (15 rats) and control group (5 rats). SAH was induced by injection of autologous arterial blood into the cisterna magna. The density of Monoaminergic innervation in middle cerebral arteries of control rats and those of rats sacrificed on day 2?7?14 after SAH were studied with immunoperoxidase ABC technique. Exploying tyrosine-hydroxylase(TH)as label. Results: The immunopositive fibers with the brown linear structures were observed in the middle cerebral artery in the rats of both SAH and control groups. Monoaminergic nerve fibers were less dense in middle cerebral ateries of rats of SAH than those of control rats(P<0.05).Conclusion:It suggest that the sympathetic nerve might play an important role in the maintenance and development of cerebral vasospasm after SAH.