促肾上腺皮质激素释放因子受体2在人脑胶质瘤中表达及其临床意义

目的探讨促。肾上腺皮质激素释放因子受体2（conicotmpin-releasing factor receptor type2,CRFR2）在人脑胶质瘤中表达及其临床意义。方法随机收集35例手术切除的胶质瘤组织标本及培养人类胶质母细胞瘤株U87,进行CRFR2免疫组织化学染色,分别取5例泌乳素垂体腺瘤组织标本作为阳性对照及5例正常脑组织作为阴性对照。结果CRFR2着色于肿瘤细胞膜,在不同类型、级别的脑胶质瘤中均有着色,且随肿瘤病理级别增高而表达增高,差异有统计学意义（P〈0．05）,在正常脑组织中呈阴性表达,U87细胞株中存在CRFR2＇高表达。结论CRFR2可能参与胶质瘤恶性生物学行为形成,但其具体作用及机制不详。

EXPRESSION AND CLINICAL SIGNIFICANCE OF CRFR2 IN HUMAN GLIOMA

Objective To explore the expression of corticotropin-releasing factor receptor type 2 （CRFR2） and the clinical significance in human glioma. Methods The expression＇ of CRFR2 was detected by immunohistochemistry in 35 patients with glioma, 5 normal brain tissues and 5 prolactin adenoma tissues. The expression of CRFR2 in the glioblastoma cell lines U87 was detected by immunocytochemistry. Results CRFR2 was stained in the tumor cell membrane in different types and grades of glioma. The positive expression of CRFR2 was increasead with glioma grades （ P 〈0.05 ）, but negative expression in the normal brain tissues. The positive expression of CRFR2 were found in U87 cell lines. Conclusion The expression of CRFR2 related to the malignant characteritic of gliomas, and might cooperate with each other in the tumor proliferation and progression. The results suggest that CRFR2 may be related! to pathological diagnosis and prognosis evaluation for malignant gliomas. However, the mechanism responsible for them in tumorigenesis and the biological functions merit further evaluation.