NanoString数字基因定量技术在生物医学领域的应用进展

NanoString数字化基因分析系统是最新的多重基因定量检测技术.此技术可直接检测条形码探针标记的单个mRNA转录子,并通过数字计数进行定量;它仅需100 ng的RNA即可对逾八百个特异的mRNA转录子进行准确的定量;它无需酶促和扩增过程,其检测的敏感性和准确性与实时荧光定量PCR（RQ-PCR）技术相当.近年来,NanoString技术的特点及优势使其被越来越广泛地应用到生物医学前沿领域,包括高通量基因表达结果验证、基因表达谱研究、基因调控网络研究、临床疾病分子分型及诊断预后等领域.

Application of NanoString nCounter System in biomedical fields

NanoString nCounter Analysis System is a newly developed gene expression detection platform that directly measures multiplexed mRNA levels through digital counting of individual mRNA transcripts.This technology uses as little as 100 ng of RNA and can obtain accurate gene quantitative data from up to 800 genes in one reaction.It requires no reverse transcription,enzymes and amplifications,and its sensitivity and accuracy are comparable to real time quantitative PCR.NanoString technology has been more and more extensively used in frontiers of biomedical research and clinics such as in validation of data from high-throughput platforms,gene expression profiling,gene regulatory network,molecular subtyping,diagnosis and prognosis of diseases.