LMWH对裸鼠胃癌原位模型生长、转移及趋化因子SDF-1表达的影响

【目的】探讨低分子肝素（LMWH）对裸鼠胃癌原位模型生长、转移及趋化因子SDF-1表达的影响。【方法】应用原位移植方法建立裸鼠胃癌原位模型，术后7d将裸鼠随机分为3组：对照组（生理盐水）、化疗组（5-氟尿嘧啶）、LMWH组（低分子肝素钙），分别每周注药2次，共5周，第6周处死动物，观察裸鼠胃癌原住模型肿瘤转移及生长情况。采用RT—PCR技术检测胃肿瘤组织SDF-1mRNA表达水平。【结果】化疗组、LMWH组肿瘤体积较对照组明显缩小，差异有统计学意义（P〈0．05）；LMWH组胃癌原位模型肿瘤转移率（20％）低于对照组（70％），差异有统计学意义（P〈0．05），而与化疗组之间差异无统计学意义（P〉o．05）；LMWH组SDF-1mRNA表达均较对照组和化疗组低，差异有统计学意义（P〈0．05）。【结论】LM—wH可抑制裸鼠胃癌原位模型的肿瘤生长及远处转移，其部分机制可能与降低肿瘤sDF_1的表达有关。

Effect of LMWH on Growth and Metastasis of Orthotopic Transplantation Gastric Tumor Model in Nude Mice and the Expression of SDF-1

Objective] To explore the effect of low-molecular weight heparin（LMWH） on tumor metasta- sis and growth and the expression of SDF-1 in orthotopic transplantation gastric tumor model of nude mice. Methods] The models of orthotopic transplantation gastric cancer in nude mice was established by orthotopic transplantation. All nude mice were randomly divided into control group（normal saline）, chemotherapy group （5-fluorouracil） and LWMH group（LWMH）. The treatment duration was twice a week for 5 weeks. The animals were sacrificed at 6 weeks. The tumor growth and metastasis of orthotopic gastric cancer in nude mice were observed. RT-PCR was used to detect the expression of SDF-1 mRNA in gastric cancer tissues. Re sults] Compared with control group, the tumor size in chemotherapy group and LMWH group were reduced obviously, and there was significant difference（ P 〈0.05）. The tumor metastasis rate in LMWH group was lower than that in control group（20% vs. 70%）, and there was significant difference between LMWH group and control group（ P 〈0.05）, but there was no difference between LMWH group and chemotherapy group（ P 〉0.05）. The expression of SDF-1 mRNA in LMWH group was lower than that in control group and chemo therapy group, and there was significant difference（ P 〈0.05）. Conclusion] LMWH can inhibit the tumor growth and distant metastasis of orthotopic transplantation gastric cancer in nude mice. The mechanism may be associated with the decreasing of SDF-1 expression.