HB-H-6树脂吸附特异性的研究

目的 考察HB-H-6树脂对不同相对分子质量蛋白质和不同结构化学物质的吸附性能,进而研究其吸附特异性.方法 采用经过处理后的HB-H-6树脂对5种不同相对分子质量的蛋白质和不同结构的化学物质蛋白质、糖类、脂类（人体血浆中的蛋白质、糖类、脂类和人血清白蛋白（HAS）、右旋糖酐（Dextran）2部分吸附实验）进行体外静态吸附实验,计算吸附率并进行比较.结果 体外静态吸附实验结果表明,HB-H-6树脂对5种不同相对分子质量的蛋白质肌红蛋白（Myo,16700）、鸡卵清蛋白（OVA,44000）、人血清白蛋白（HAS,66 200）、β-半乳糖苷酶（β-gal,130 000）、免疫球蛋白G（IgG,150 000）的平均吸附率分别为（0.00±.0.33）％、（8.02±1.23）％、（43.19±2.31）％、（34.25±1.07）％、（0.00±0.69）％.HB-H-6树脂对血浆中不同结构的化学物质总蛋白、白蛋白、球蛋白、葡萄糖、甘油三酯及胆固醇的平均吸附率分别为（11.18±0.72）％、（10.74±0.66）％、（11.74±1.22）％、（7.17±0.12）％、（1.06±1.04）％、（3.05±0.65）％.HB-H-6树脂对右旋糖酐（Dextran,70 000）体外静态吸附实验的平均吸附率为（5.44±1.46）％,明显低于对HAS的平均吸附率（（43.19±2.31）％）.结论 HB-H-6树脂对不同相对分子质量的蛋白质的吸附率差异明显,对相对分子质量在6×104～1×105之间的蛋白质的吸附率较高.HB-H-6树脂对不同结构的化学物质蛋白质、糖类及脂类的吸附率也有明显不同.其中,HB-H-6树脂对血浆中蛋白质的吸附率高于糖类和脂类;另外,HB-H-6树脂对不同结构的化学物质HAS和Dextran的吸附率也差异明显.由此表明,HB-H-6对不同相对分子质量蛋白质和不同结构化学物质的吸附有吸附特异性.

Study on adsorption specificity of HB-H-6 resin

Objective The aim of this article was to deeply study the effects of different molecular weight of proteins and different structures of chemical substances on the adsorption specificity of HB-H-6 resin.Methods HB-H-6 resin was adopted to adsorb 5 different molecular weight proteins and different structural chemical substances including proteins,saccharides,human serum albumin （HAS）,dextran and lipid,and then underwent static adsorption experiments in vitro.The adsorption rates of different structural chemical substances were analyzed from two experiments and the results were compared.Results The experiment results of HB-H-6 resin adsorption showed that the average adsorption rates of 5 different molecular weight proteins,myoglobin （Myo,16 700）,ovalbumin （OVA,44 000）,HAS （66 200）,β-gal （130 000） and IgG （150 000）,were significantly different：（0.00±0.33）％,（8.02± 1.23）％,（43.19±2.31）％,（34.25±1.07）％ and （0.00±0.69）％.In the studies on adsorption of different structural chemical substances,the average adsorption rates of different structural chemical substances proteins,saccharides,lipid were significantly different：the absorption rates of plasma total protein,albumin,globulin,glucose,triglyceride and cholesterol groups were：（11.18±0.72）％,（10.74±0.66）％,（11.74± 1.22）％,（7.17±0.12）％,（1.06± 1.04）％,（3.05± 0.65）％.The absorption rates of HAS and dextran groups were：（43.19±2.31）％ and （5.44±1.46）％.Conclusion In conclusion,the proteins＇ molecular weight of best adsorption condition is from 66 Ku to 130 Ku.The average adsorption rates of different structural chemical substances proteins,saccharides,lipid are significantly different.The average adsorption rates of proteins are higher than that of saccharides and lipid.It shows that HB-H-6 resin has adsorption specificity on different molecular weight proteins and different structural chemical substances.