瘢痕疙瘩和增生性瘢痕表皮异常的实验研究

目的 探讨瘢痕疙瘩和增生性瘢痕的表皮异常。方法 采用免疫组织化学方法,检测腱糖蛋白C(Tn-C),角蛋白16(CK-16)和增殖相关核抗原Ki-67蛋白在瘢痕疙瘩、增生性瘢痕和正常成人表皮中的表达。取正常成人皮肤组织RNA,构建正义、反义Tn-C mRNA探针,运用原位杂交技术,观测瘢痕疙瘩、增生性瘢痕和正常皮肤表皮中Tn-C mRNA的表达。结果 瘢痕疙瘩、增生性瘢痕表皮角质形成细胞增生明显高于正常皮肤。Tn-C mRNA在瘢痕疙瘩表皮的表达明显高于其在增生性瘢痕及正常皮肤表皮中的表达。CK-16,Ki-67蛋白在瘢痕疙瘩和增生性瘢痕表皮中表达增加。结论 瘢痕疙瘩和增生性瘢痕的表皮存在增生分化异常,尤以瘢痕疙瘩更为明显。

A study of the abnormalities of human epiderm in keloids and hypertrophic scars

Objective To investigate the abnormalities of human epiderm in keloids and hypertrophic scars Methods Biopsies from ten untreated keloids (duration of disease 3 30 years) and ten hypertrophic scars (duration of disease 6 10 months) and five normal adult skin specimens Total RNA was isolated from normal adult skin A cDNA fragment (base 5941-6481bp ) of the full length human Tenascin C cDNA was synthesized by polymerase chain reaction and subcloned in pGEM T easy Dioxigen labeled anti sense and sense probles were synthesized by using a Sp6/T7 in vitro RNA synthesis kit in the present of Dig UTP In situ hybridization was performed on 4% paraformaldehyde fixed and wax embedded sections of keloids and hypertrophic scars NBT NCIP was used in color detection Immunohistochemical procedure The sections were incubated with antibodies (anti Tenascin C, anti CK 16 and anti Ki 67 ) Ultrasensitive Streptavidin Peroxidase staining was performed following established procedures Results The study show that the proliferation of epidermal keratinocytes in keloids and hypertrophic scars is very clear The expressions of Tenascin C mRNA in keloids epidermal keratinocytes markedly increased in contrast with epidermal keratinocytes of hypertrophic scars and adult skin The CK 16 and Ki 67 stainings significantly enhanced in the epidermal keratinocytes of keloids and hypertrophic scars Conclusions The different expressions of Tenascin C, CK 16 and Ki 67 among normal adult skin, keloids and hypertrophic scars show the abnormalities of epidermal keratinocytes proliferation and differentiation in keloids and hypertrophic scars