清心安神方含药血清对离体心室肌细胞内向钾电流(Ik1)的影响 |
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引用本文: | 方居正,;丰俊鹏,;杜来义.清心安神方含药血清对离体心室肌细胞内向钾电流(Ik1)的影响[J].中医临床研究,2014(12):147-148. |
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作者姓名: | 方居正 ;丰俊鹏 ;杜来义 |
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作者单位: | [1]河南省平顶山市中医院,河南平顶山467000; [2]河南中医学院,河南郑州450000; [3]武汉市中医院,湖北武汉430010 |
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基金项目: | 本项目为河南省2013年度科技攻关项目,项目编号:132102310403,膜片钳技术在清心安神法影响心肌细胞离子通道电流实验中的应用. |
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摘 要: | 目的:观察清心安神方含药血清对豚鼠离体心室肌细胞内向钾电流(Ik1)。方法:分离豚鼠的心室肌细胞,采用全细胞膜片钳技术电流钳模式记录动作电位,观察含40%的空白血清及含药血清的细胞外液灌流对 Ik1的影响。结果:清心安神方含药血清对豚鼠心室肌细胞内向钾电流(Ik1)影响:40%含药血清组对IKl内向电流成分影响为:40%含药血清组从(-30.68±10.39)pA/pF抑制为(-21.03±3.47)pA/pF(n=7 P〈0.05)。结论:清心安神方含药血清对大鼠心室肌细胞IKl内向成份有抑制作用,呈浓度依赖性。
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关 键 词: | 清心安神方 血清药理学 膜片钳 内向钾电流 心律失常 |
Influence of serum containing heart-clearing and spirit-calming decoction on inward rectifier potassium channel current in isolated ventricular myocytes |
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Abstract: | Objective:To observe the influence of serum containing Heart-Clearing and Spirit-Calming Decoction on inward rectifier potassium channel current in ventricular myocytes isolated from Rat. Methods:Record the action potential by whole patch clamp technique and currentc lamp m ode. Observe the r espective influence of extracellular fluid perfusion of 40%blank serum and drug serum on IK1. Results:At the test with voltage of-100mV,the inward component of IK1 in the 40%drug group was decreased from(-30.68±10.39)pA/pF to(-21.03±3.47)pA/pF(n=7 P〈0.001). Conclusion:40%serum containing Heart-Clearing and Spirit-Calming Deooction can decrease the IK1. It was concentration dependent. |
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Keywords: | Heart-Clearing and Spirit-Calming Decoction Serum pharmacology Patch clamp technique inward rectifier potassium channel current arrhythmia |
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