| 顺铂对人卵巢癌CAOV3细胞端粒酶活性和凋亡的影响 |
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| 引用本文： | 杨平,初照成,王实,孙莉莉,周郦楠,张丽雁,张广新. 顺铂对人卵巢癌CAOV3细胞端粒酶活性和凋亡的影响[J]. 中国全科医学, 2005, 8(11): 896-897 |
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| 作者姓名： | 杨平 初照成 王实 孙莉莉 周郦楠 张丽雁 张广新 |
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| 作者单位： | 1. 110101,辽宁省沈阳市,辽宁中医学院职业技术学院
2. 辽宁省血栓病中西医结合医疗中心 |
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| 摘 要： | 目的研究顺铂作用于卵巢癌CAOV3细胞后，端粒酶活性、细胞凋亡和细胞周期变化与细胞耐药性的关系。方法用顺铂处理卵巢癌CAOV3细胞，端粒酶重复序列扩增(TRAP)一银染法测定端粒酶活性，流式细胞仪观察细胞凋亡和细胞周期的改变。结果顺铂(DDP)处理卵巢癌CAOV3细胞后，CAOV3细胞端粒酶活性受到抑制，细胞周期受阻于G2／M期，未见细胞凋亡峰改变。结论CAOV3细胞对顺铂的耐药性通过多因素起作用，DDP对端粒酶活性具有抑制作用，可以改变细胞周期，但对细胞凋亡无明显影响。
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| 关 键 词： | 顺铂 卵巢癌细胞 端粒酶 凋亡 |
| 修稿时间： | 2005-02-20 |
The Effect of Cisplatin on Telomerase Activity and Apoptosis in CAOV3 Cells |
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| YANG Ping,CHU Zhao-cheng,WANG Shi,et al.. The Effect of Cisplatin on Telomerase Activity and Apoptosis in CAOV3 Cells[J]. Chinese General Practice, 2005, 8(11): 896-897 |
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| Authors: | YANG Ping CHU Zhao-cheng WANG Shi et al. |
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| Affiliation: | YANG Ping,CHU Zhao-cheng,WANG Shi,et al.Occupational and Technological College,Liaoning Traditional Chinese College,Liaoning 110101,China |
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| Abstract: | Objective To investigate the drug resistance to cisplatin of the ovarian cancer CAOV3 line and the relationships between the drug resistance and telomerase activity,apoptosis,cell cycle.Methods CAOV 3 cells were treated with cisplatin.Telomerase activities were assayed by the method of telomeric repeat amplification protocol (TRAP)-silver straining.The change of cell cycle and apoptosis were analyzed by flow cytometer.Results It was found that telomerase activity of CAOV 3 cells inhibition and cell cycle accumulation in G2/M phase without apoptosis phase during the treatment.Conclusion The drug resistance to cisplatin of ovarian cancer CAOV 3 line is effected by several agent,which is not changed directly the inhabition of cisplatin to CAOV 3 cells or induce the apoptosis of cell CAOV 3 line.The apoptosis induced by cisplatin closely related to cell cycle. |
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| Keywords: | Cisplatin Ovarian cancer Telomerase Apoptosis |
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