CD31、CD34和CD105蛋白在食管鳞状细胞癌组织微血管中的表达及意义

目的探讨CD31、CD34和CD105蛋白表达在食管鳞状细胞癌（ESCC）发生、发展中的作用。方法应用免疫组化SP法对50例ESCC和10例正常食管黏膜组织标本进行标记染色，按Weidner法计算三种蛋白标记物的肿瘤组织微血管密度（MVD）。结果在ESCC及正常食管黏膜组织中MVD-CD31、MVD—CD34及MVD-CD105均依次降低，组间比较尸〈0．01；MVD-CD31、MVD—CD34与ESCC的浸润深度及淋巴结转移密切相关（P〈0．05），MVD—CD105与ESCC的TNM分期、浸润深度及淋巴结转移密切相关（P〈0．01）；ESCC组织中MVD—CD31、MVD—CD。均显著高于MVD-CD105（P〈0．01）。结论CD31、CD34和CD105蛋白表达在ESCC发生、发展和转移中起重要作用；联合检测三者可望为判断ESCC发展及利用血管抑制剂治疗提供理论依据。

Expression and meanings of CD31, CD34 and CD105 protein in the tissue microvessel of esophageal squamous cell carcinoma

Objective To investigate the effects of the expression of CD31 , CD34 and CD105 protein in the pathogenesis and development of esophageal squamous cell carcinoma（ESCC）. Methods All the specimens＇segments were stained by immunohistochemical SP method in 50 cases of ESCC tissue and 10 cases of normal esophageal epithelium, and then the microvessel density（MVD） marked by three protein were counted by the Weidner＇s method. Results The expression of MVD-CD31 ,MVD-CD34 and MVD-CD105 decreased by turns in ESCC and normal esophageal epithelium specimens. There was a significant difference among the groups （P 〈 0.01 ）. The MVD-CD31 and MVD-CD34 were closely correlated with infiltration and lymphatic metastasis in ESCC （ P 〈 0.05 ）. The MVD-CD105 was closely correlated with the TNM grade, infilatration and lymphatic metastasis in ESCC （P 〈 0.01 ）. MVD-CD31 and MVD-CD34 were significantly higher than MVD-CD105 in ESCC tissue（P 〈0.01）. Conclusions CD31, CD34 and CD105 protein may play important roles in the carcinogenesis, metastasis and infiltration of ESCC. United examination of these three protein is expected to determine the carcinogenesis of ESCC and provide theoretical evidence to the therapy by using vascular inhibitor.