| 人成骨细胞胰岛素受体底物的表达 |
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| 引用本文： | 黄秋霞,周后德,廖二元,杜巍. 人成骨细胞胰岛素受体底物的表达[J]. 中国医师杂志, 2005, 7(5): 597-599 |
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| 作者姓名： | 黄秋霞 周后德 廖二元 杜巍 |
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| 作者单位： | 1. 广东省东莞市人民医院内分泌科
2. 中南大学湘雅二医院代谢内分泌研究所,湖南,长沙,410011 |
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| 基金项目： | 国家自然科学基金资助项目(30400218) |
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| 摘 要： | 目的 观察人成骨肉瘤MG-63细胞和正常人成骨细胞(human osteoblast-like cells HOB)胰岛素受体底物(insulin reoeptor substrate IRS)的表达。方法 半定量RT-PCR检测IRS mRNA表达，免疫共沉淀检测IRS蛋白表达。结果 人成骨肉瘤MG-63细胞和正常人成骨细胞均有IRS-1、-2、-3、-4mRNA和蛋白质的表达，以IRS-1的表达丰度最高，IRS-4表达丰度最低。其中IRSmRNA和蛋白质在MG-63细胞中的表达丰度较在正常人戍骨细胞中的表达丰度稍高。结论 人成骨肉瘤MG-63细胞和正常人成骨细胞均可表达IRS mRNA和蛋白质。且IRS不同成员在同一细胞中的表达丰度不同。
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| 关 键 词： | 胰岛素受体底物 人成骨细胞 MG-63细胞 定量RT-PCR检测 人成骨肉瘤 表达丰度 IRS-1 mRNA表达 cells 免疫共沉淀 IRS-4 蛋白质 蛋白表达 正常人 |
| 修稿时间： | 2004-12-14 |
The Expression of Insulin Receptor Substrate in Human Osteoblast |
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| HUANG Qiu-xia,ZHOU Hou-de,LIAO Er-yuan,et al.. The Expression of Insulin Receptor Substrate in Human Osteoblast[J]. Journal of Chinese Physician, 2005, 7(5): 597-599 |
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| Authors: | HUANG Qiu-xia ZHOU Hou-de LIAO Er-yuan et al. |
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| Affiliation: | HUANG Qiu-xia,ZHOU Hou-de,LIAO Er-yuan,et al. Institute of Endocrinology and Metabolism,The Second Xiangya Hospital,Central South University,Changsha 410011,China |
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| Abstract: | Objective To determine the expression of insulin receptor substract(IRS) family in human osteosarcoma cell line MG-63 and cultured normal human osteoblast-like cells (HOB). Methods The mRNA and protein expression of IRS family was measured using semi-quantitative RT-PCR and western blot analysis, respectively. Results MG-63 cells and HOB had the mRNA and protein expressions of IRS-1,-2,-3, and -4. Among IRS family, the expressions levels of IRS-1 mRNA and protein were the highest, and those of the IRS-4 mRNA and protein were the lowest in MG-63 cell line and HOB. Conclusion MG-63 and HOB can express the mRNA and protein of IRS family members, and the expressional levels of IRS family members were different. |
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| Keywords: | Human osteoblast Human osteosarcoma cell line MG-63 Insulin receptor substrate Insulin receptor |
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