B Cell Hybridoma Presents Both B-Cell and T-Cell Epitopes for Stimulating Antibody Production Via CD23 Pathway |
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| Authors: | Robert Schmaltz Yan-Yu Wang Qing-Xian Kung Fu-Tong Liu Thomas Petro Swey-Shen Chen |
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| Institution: |
a Department of Veterinary Science, University of Nebraska-Lincoln, IANR, Omaha, NE
b Allergy Research Section, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA
c Department of Oral Biology, University of Nebraska Medical Center, Omaha, NE
d Department of Pathology and Microbiology, The Scripps Research Institute, La Jolla, CA |
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| Abstract: | CD23+ B cell hybridoma 17A11, pulsed with IgE:TNP-KLH triggered IgA, IgG, and IgE antibody production via CD23-mediated presentation. Prior anti-CD23 treatment abrogated 95% of the humoral antibody responses. Both B and T cell epitopes were presented by 17A11. B cell epitopes as recognized by IgG but not T cell epitopes were sensitive to treatment with 0.2 M acetic acid. Efficacy of antigen presentation via CD23 on 17A11 was comparable to that mediated via surface immunoglobulins (sIg) on a CD23 negative 4.5 parental fusion partner B cell line. This is the first demonstration that IgE:TNP-KLH pulsed B cell hybridomas present both B-and T-cell epitopes in stimulating IgA, IgG, and IgE antibody production, and raise a pertinent issue whether IgE antibodies produced under pathophysiological conditions may serve as positive feedback signal for sustaining production of different classes of antibodies. |
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