Accuracy of radiographic pixel linear analysis in detecting bone loss in periodontal disease: Study in diabetic rats |
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| Authors: | Carlos Roberto Emerenciano Bueno,Doris Hissako Sumida,Marco Antô nio Hungaro Duarte,Ronald Ordinola-Zapata,Mariane Maffei Azuma,Gustav Guimarã es,Tiago Novaes Pinheiro,Luciano Tavares Angelo Cintra |
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| Affiliation: | 1. Department of Preventive and Restorative Dentistry, Endodontic Section, School of Dentistry, São Paulo State University (Unesp), São Paulo, SP, Brazil;2. Department of Basic Science, School of Dentistry, São Paulo State University (Unesp), São Paulo, SP, Brazil;3. Department of Dentistry, Endodontics and Dental Materials, School of Dentistry, University of São Paulo – USP, Bauru, SP, Brazil;4. Division of Endodontics, University of Minnesota, School of Dentistry, Minneapolis, MN, USA;5. Department of Cariology, Restorative Sciences and Endodontics, University of Michigan, Ann Arbor, MI, USA;6. Department of Periodontics, University Center São Lucas, School of Dentistry, Porto Velho, RO, Brazil;7. Department of Oral Pathology and Oral Medicine, Dental School of Amazonas State University, Manaus, AM, Brazil;8. Dental Assistance Center for Disabled Persons (CAOE) of the São Paulo State University (Unesp), School of Dentistry, Araçatuba, SP, Brazil;9. Department of Endodontics, State University of Northern Paraná (UENP), School of Dentistry, Jacarezinho, PR, Brazil |
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| Abstract: | IntroductionPeriodontitis, a complex infectious disease that may lead to irreversible loss of periodontium, is considered a predisposing agent for developing insulin resistance due to the release of inflammatory mediators, showing a bilateral relationship with diabetes mellitus. The investigation of periodontal disease requires a clinical approach and complete intraoral radiographs, even with increasing concerns about radiation exposure. Thus, this study assesses pixel linear analysis accuracy using digital radiography via Digora® in detecting alveolar bone destruction in diabetic rats with periodontal disease.Methodology40 rats were divided into groups (n = 10): control (C), rats with periodontal disease (PD), experimental diabetic rats (ED), experimental diabetic rats with periodontal disease (ED-PD). Diabetes was induced by streptozotocin and periodontal disease by periodontal ligature. After 30 days, maxillae bone destruction was obtained by linear analysis of vertical bone loss using digital radiography and then assessed by micro-CT and histology. Data were analyzed by ANOVA and Tukey’s test (p < 0.05).ResultsRadiographic, micro-CT and histological analysis presented accurate and similar results. PD and ED-PD groups showed higher bone destruction than C and ED groups (p < 0.05). Moreover, the ED-PD group had higher bone loss than the PD group (p < 0.05).ConclusionThe pixel linear analysis via digital radiography was an accurate, low-cost alternative in detecting alveolar bone loss in this rat model. Micro-CT and histological analysis may also be used to obtain linear measures to assess and compare periodontal bone destruction in diabetic rats. |
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| Keywords: | Experimental diabetes mellitus Histology Periodontal disease Radiography Emission-computed tomography |
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