| 青藤碱清除氧自由基和抗脂质过氧化作用 |
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| 引用本文： | 刘刚,王辉,张先洲,罗顺德.青藤碱清除氧自由基和抗脂质过氧化作用[J].中草药,2006,37(1):84-87. |
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| 作者姓名： | 刘刚 王辉 张先洲 罗顺德 |
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| 作者单位： | 武汉大学人民医院,药学部,湖北,武汉,430060 |
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| 摘 要： | 目的研究青藤碱体外清除氧自由基与抗脂质过氧化的作用。方法超氧阴离子自由基(O2÷)由邻苯三酚自氧化法产生,采用F en ton体系产生羟自由基(.OH),研究青藤碱对O2÷和.OH的清除作用;用硫代巴比妥酸(TBA)法测定VC-F e2 系统诱导大鼠肝匀浆及肝线粒体产生的脂质过氧化物丙二醛(M DA),研究青藤碱抗脂质过氧化的作用。结果青藤碱能清除.OH和O2÷,其EC50分别为1.35和0.136 m g/mL,且呈浓度依赖关系,青藤碱能抑制大鼠肝匀浆自氧化及VC-F e2 系统诱导引起的脂质过氧化。结论青藤碱具有清除氧自由基和抗脂质过氧化的作用,对肝线粒体也有保护作用。
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| 关 键 词： | 青藤碱 氧自由基 脂质过氧化 |
| 文章编号： | 0253-2670（2006）01-0084-04 |
| 收稿时间： | 2005-04-02 |
| 修稿时间： | 2005-04-02 |
Effect of sinomenine on scavenging oxygen free radical and anti-lipid peroxidation |
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| LIU Gang,WANG Hui,ZHANG Xian-zhou and LUO Shun-de.Effect of sinomenine on scavenging oxygen free radical and anti-lipid peroxidation[J].Chinese Traditional and Herbal Drugs,2006,37(1):84-87. |
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| Authors: | LIU Gang WANG Hui ZHANG Xian-zhou and LUO Shun-de |
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| Institution: | Department of Pharmacy,Renmin Hospital of Wuhan University,Wuhan 430060,China;Department of Pharmacy,Renmin Hospital of Wuhan University,Wuhan 430060,China;Department of Pharmacy,Renmin Hospital of Wuhan University,Wuhan 430060,China;Department of Pharmacy,Renmin Hospital of Wuhan University,Wuhan 430060,China |
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| Abstract: | Objective To study the effects of sinomenine on scavenging oxygen free radical and anti-lipid peroxidation in vitro. Methods Superoxide anion free radical(O_2~÷) induced by the auto-oxidation of pyrogallol and hydroxyl free radical(OH) induced by Fenton system were determined by spectrophotometry.The inhibition on homogenate and mitochondrial lipid peroxidation,malondialdehyde(MDA),of rat liver induced by VC-Fe(2+) system was measured by thiobarabituric acid(TBA) colorimetric method.Results Sinomenine could significantly scavenge O2~÷ and ·OH,it's EC_(50) were 1.35 and 0.136 mg/mL respectively.Sinomenine could also remarkably inhibit the lipid peroxidation of rat liver homogenate and mitochondria induced by auto-oxidation and VC-Fe(2+) system.The scavenging and inhibitory effect exhibited in a concentration-dependent manner.Conclusion The Results indicate that sinomenine possesses the effect of scavenging oxygen free radicals and anti-lipid peroxidation,which shows the protection of liver mitochondria |
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| Keywords: | sinomenine oxygen free radical lipid peroxidation |
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