Anti-inflammatory action of alpha-melanocyte stimulating hormone (alpha-MSH) in anti-CD3/CD28-mediated spleen and CD4(+)CD25(-) T cells and a partial participation of IL-10 |
| |
Authors: | Chang Sung Ho Jung Eun Jung Lim Dong Gyun Park Youn Hee Wee Yu Mee Kim Jin Hee Kim Yang Hee Choi Monica Young Koo Sun Kyung Choi Kang Duk Han Duck Jong Kim Song Cheol |
| |
Institution: | Department of Surgery and Asan Institute for Life Sciences, Ulsan University College of Medicine & Asan Medical Center, Songpa-Gu, Seoul, Republic of Korea. |
| |
Abstract: | alpha-Melanocyte stimulating hormone (alpha-MSH) has been shown to inhibit the production and the effects of pro-inflammatory cytokine by inflammatory cells in innate immunity. We have determined whether alpha-MSH inhibits anti-CD3/CD28-mediated spleen cells and CD4(+)CD25(-) T cells proliferation and its mechanism of action. The proliferation of anti-CD3/CD28-mediated spleen cells and CD4(+)CD25(-) T cells markedly were suppressed by 50-100 nM and 5-100 nM alpha-MSH, respectively. alpha-MSH (100 nM) increased the production of the anti-inflammatory cytokine IL-10 and decreased the production of the pro-inflammatory cytokine IL-2 and IFN-gamma from CD4(+)CD25(-) T cells. Moreover, anti-IL-10 blocking Ab decreased the inhibitory effects of anti-CD3/CD28-mediated spleen cells and CD4(+)CD25(-) T cells proliferation by alpha-MSH, indicating a partial participation of IL-10 in its mechanism of inhibitory action. These results suggest that alpha-MSH may be useful for treatment of autoimmune diseases and transplantation involving innate and adaptive immunity. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|