| 单克隆抗体亲和层析法纯化SEB及其活性鉴定 |
| |
| 引用本文： | 张靖,王惠芳,高川,宋云扬. 单克隆抗体亲和层析法纯化SEB及其活性鉴定[J]. 中国免疫学杂志, 2006, 22(10): 944-947 |
| |
| 作者姓名： | 张靖 王惠芳 高川 宋云扬 |
| |
| 作者单位： | 防化研究院第四研究所,北京,102205 |
| |
| 摘 要： | 目的：采用亲和层析法获取高纯度的金黄色葡萄球菌肠毒素B（Staphylococcal enterotoxin B，SEB），并对其进行活性分析。方法：采用正辛酸沉淀法纯化了一株抗SEB的单克隆抗体（1D2），与CNBr活化的Sepharose4B偶联制成亲和层析柱纯化SEB，并对其超抗原活性和抗原活性进行鉴定。结果：与离子交换纯化法和市售的标准品相比较，亲和层析法纯化的SEB纯度高，纯化的效率为60．71％。同时，纯化后的SEB具有良好的抗原性和超抗原活性。结论：单克隆抗体亲和层析法可以高效率地纯化高纯度的SEB。
|
| 关 键 词： | 单克隆抗体 亲和层析 |
| 文章编号： | 1000-484X（2006）10-0944-04 |
| 收稿时间： | 2005-10-29 |
| 修稿时间： | 2005-10-292006-03-06 |
Purification of SEB by McAb affinity chromatography and activity analysis |
| |
| ZHANG Jing,WANG Hui-Fang,GAO Chuan,SONG Yun-Yang. Purification of SEB by McAb affinity chromatography and activity analysis[J]. Chinese Journal of Immunology, 2006, 22(10): 944-947 |
| |
| Authors: | ZHANG Jing WANG Hui-Fang GAO Chuan SONG Yun-Yang |
| |
| Affiliation: | Research Institute of Chemical Defense, Beijing 102205, China |
| |
| Abstract: | Objective:To prepare highly purified Staphylococcal enterotoxin B(SEB) by affinity chromatography and test its activities.Methods:Anti-SEB McAb(1D2) purified by precipitation method with caprylic acid was coupled to Sepharose 4B. And then the SEB was isolated using an affinity chromatography column. In addition, we analyzed the superantigen activity and antigen activity of SEB.Results:The purification efficiency of SEB was 60.71% by affinity chromatography. Its purity was higher than those of standard preparation and the SEB purified by ion change chromatography. At the same time, the purified SEB by affinity chromatography possesses favourable activities of superantigen and antigen.Conclusion:McAb affinity chromatography could be used for purification of SEB with high efficiency. |
| |
| Keywords: | SEB |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
