小肠浆肌膜腔内自体脾组织移植动物模型的建立与意义

目的探讨增加自体脾组织移植量的方法,提高自体移植脾组织的功能。方法贵州小型香猪3头,经肌注麻醉后剖腹切除脾脏,取脾脏的1/2约150g,切成1mm×1mm×1mm小块备用。在距Treitz韧带50cm处,切取保留肠系膜血管的空肠30cm旷置,再吻合肠管恢复肠腔通道,缝闭肠系膜裂口。旷置空肠等分2段,去除肠粘膜,缝闭一端,将约50g脾组织块分别植于2段肠浆肌膜腔内,缝闭另一端口,妥善固定于肠系膜上。大网膜内移植30g脾组织作为对照观察。结果4个月饲养期间内无肠梗阻发。3段肠浆肌膜腔内脾组织再生良好,组织结构与大网膜内移植脾组织无明显差别,2段再生较差,1段形成脓肿。结论肠浆肌膜腔内移植脾组织的量较大,可作为自体脾组织的移植术式。

Splenic autotransplantation to small intestine without mucous membrane in swine

Objective To explore more effective measu re for splenic autotranspla ntation to enhance the function of splenic autotransplanted tissue. Met hods Spl enectomy on 3 Guizhou swines was first performed in sterile conditions under gen eral anesthesia by submuscular injection of 3% pentobarbital. A half of the spl e en about 150 g from each animal was cut into pieces (1 mm 1 mm 1 mm) for transpl antation. Two 15-cm long segments of proximal jejunum at the distance of 50 cm f rom Treitz ligment were isolated on their mesentery with intact circulation. Bo w el continuity was re-established by end-to-end anastomosis and the mesentery was sutured. The mucosal layer was removed from the isolated segments of small int e stine and splenic pieces were implanted into the prepared isolated seromuscular loops of small intestine. The ends of the isolated bowel segments were closed. T he bowel segments were fixed to the nearby mesentery to prevent torsion of their vascular pedicles. The splenic pieces about 30 g were implanted in greater ome n tum as control. Results No intestinal obstruction was observe d in 4 months afte r transplantation. Implanted pieces of splenic tissue regenerated well in 3 bow e l segments, not well in 2 bowel segments. An abscess was in 1 bowel segment wit h out regeneration of splenic tissue. Histomorphological appearance of the autotr a nsplanted splenic tissue in the isolated seromuscular loops of small intestine d id not differ from that in greater omentum. Conclusion Isolat ed seromuscular loops of small intestine may be considered as an alternative pla ce of autotransplanted splenic tissue.