基质金属蛋白酶-2及孕激素受体在子宫内膜癌组织中的表达及其意义

目的检测基质金属蛋白酶-2（MMP-2）和孕激素受体（PR）在子宫内膜癌组织中的表达，分析其在子宫内膜癌中表达的相关性以及与临床病理特征的关系，探讨MMP-2在子宫内膜癌发生、发展中的作用。方法采用免疫组化SP法检测45例子宫内膜癌和12例增生子宫内膜中MMP-2、PR的表达，以20例正常子宫内膜作为对照。结果MMP-2蛋白在正常子宫内膜、增生子宫内膜中的阳性表达率比较，差异无显著性（P〉0．05）。子宫内膜癌组织中MMP-2阳性表达率（62．2％）高于对照组（15．6％）（P〈0．05）。MMP-2在中、低分化子宫内膜癌组织中的阳性表达率（82．6％）高于高分化子宫内膜癌组织（40．9％）（P〈0．01），有肌层浸润标本中的阳性表达率（71．1％）高于无肌层浸润者（14．3％）（P〈0．05）；绝经者（65．5％）与未绝经者（56．3％）、手术病理为Ⅰ期者（52．0％）与Ⅱ～Ⅳ者（75．0％）以及有淋巴结转移者（63．6％）与无淋巴结转移者（61．8％）相比，MMP-2的阳性表达率差异无显著性。子宫内膜癌中PR的阳性表达率（55．6％）明显低于对照组（84．4％）（P〈0．05）。MMP-2与PR在子宫内膜癌中的表达呈负相关（P=0．0048，r=-0．42）。结论MMP-2在子宫内膜癌中呈高表达，可能在其进展中有重要作用，PR表达降低可能是MMP-2表达升高的原因；检测MMP-2在子宫内膜癌中的表达，有助于子宫内膜癌预后的判断。

Expression of matrix metalloproteinase-2 and progesterone receptor in endometrial carcinoma

Objectives To detect the expression of matrix metalloproteinase - 2 （MMP - 2） and progesterone receptor （PR） in endometrial carcinoma and to explore the relationship between them and their relationships with clinic - pathological features so as to address the role of MMP - 2 in the development of endometrial carcinoma. Methods Endometrial carcinoma tissues collected from 45 women were immunohistochemically stained by streptavi din peroxidase technique with monoelonal antibodies against MMP - 2 and PR, respectively. Twelve hyperplasia endometrium and 20 normal endometrium tissues were used as control groups. Results No significant difference of MMP - 2 expression were found between normal endometrium of proliferative phase and secretive phase （P〉0.05）. The expression of MMP - 2 in endometrial carcinoma was significantly more than in control group （ 6 2. 2 % vs 15. 6 % , P〈0.05）. The expression of MMP- 2 was significantly more in moderately and poorly differentiated carcinoma than in well differentiated carcinoma （82.6% vs 40.9 %, P〈0.01）. The expression of MMP - 2 was significantly higher in the group with myometrium invasion without myometrium invasion （71.1% vs 14.3 %, P〈0.05）. No significant difference of MMP - 2 expression was found between stage Ⅱ ～Ⅳ and stage Ⅰ. The PR expression in the carcinoma group was significantly less than in the control group （55.6% vs 84.4%, P〈0.01）. MMP - 2 expression was negatively correlated to the PR in endometrial carcinoma group （P=0. 0048, r= -0.42）. Conclusions MMP - 2 plays a role in the development of endometrial carcinoma. Down - regulation of PR might be one of the factors leading to increase of MMP - 2 in endometrial carcinoma. MMP - 2 is a potential maker to evaluate the prognosis of the endometrial carcinoma.