大鼠原位肝移植模型的技术改进

目的:探讨大鼠原位肝移植模型的技术改进.方法:选取体质量相近的SD大鼠作为供受体,分别用Kamada"二袖套法"和改良"二袖套法"(改良的方法包括:提高肝上下腔静脉的吻合质量、改进门静脉和肝下下腔静脉套管的吻合及胆总管的重建等)建立大鼠原位肝移植模型,比较两组在总的手术时间﹑无肝期时间﹑受体手术时间以及手术成功率上的差异.结果:两组手术成功率分别是78.9%(71/90)和91.7%(55/60)(P＜0.05),而两组在总的手术时间﹑无肝期时间﹑受体手术时间上无统计学差异.结论:改良的大鼠原位肝移植模型是一种简单﹑易行﹑稳定的肝移植研究模型.

Modification of technique for orthotopic liver transplantation model in rats

Objective:To investigate the modification of the technique for establishing rat model of orthotopic liver transplantation.Methods:Male Sprague-Dawley rats with similar body weights were selected as the donors and recipients.Two protocols were used for establishing orthotopic liver transplantation model in rats: one was Kamada's two-cuff method and the other was modified two-cuff method.The major modifications included the improvement of the anastomosis of superior and inferior vena cava,improvement of anastomosis between the portal vein and hepatic inferior vena cava,and the improvement of total bile duct reconstruction.The successful rate of operation,total operation time,anhepatic time,and the recipient operative time were recorded and compared in 2 groups.Results:The successful rates of the original and modified methods were 78.9%(71/90)and 91.7%(55/60),respectively(P<0.05).No differences were found between the 2 groups in the total operative time,anhepatic time,and the recipient operative time.Conclusion:The modified model is simple and easy to be made,and can serve as a stable model for the study of liver transplantation.