延迟相心肌缺血预适应机制的核医学方法研究

心肌缺血预适应是目前已知心脏保护作用最为强大的、心脏固有的自我保护机制 ,阐明预适应机制是临床合理安全地利用预适应保护作用的前提。延迟相预适应机制目前主要有 NO(一氧化氮 )假说、自由基机制、保护性蛋白的作用和 ATP敏感性钾通道作用等。引入 3H-亮氨酸 ,利用液闪技术 ,已直接从因果关系上证实保护性蛋白在预适应保护中的作用。利用 SPECT可无损伤地在活体内显示预适应心肌特点 ,而 PET则可进行预适应心肌代谢改变与心功能关系的研究。

Nuclear medicine methods in the field of studying the mechanisms of delayed phase of myocardial preconditioning

Ischemic preconditioning is the intrinsic and most potently myocardial protection we know now. To illuminate its mechanism is the foundation of rational theraputic application. Nowadays there are some theories about delayed phase of preconditioning such as nitric oxide hypothesis,free radical mechanisms, protective protein synthesis and opening of ATP sensitive potassium channels. By incorporation 3 H leucine,using liquid scintillation counter,we now know there was protective protein synthesis during preconditioning. SPECT could study the characteristics of preconditioned myocardium in vivo , and PET might further show the metabolism, energy consumption and its relationship to myocardium dysfunction.