5,7双羟色胺对不同功能运动神经元群调质CGRP配布式？…

目的 研究５,７双羟色胺（５,７－ＤＨＴ）对不同功能运动神经元（Ｍｎ）群调质ＣＧＲＰ配布式样的影响。方法 利用霍艉毒素Ｂ亚单位结合胶体取长补短地标记神经元复合化学的非荧光双标记方法。结果 大鼠比目鱼肌（慢肌）Ｍｎ（ＳＯＬ－Ｍｎ）群和趾长伸肌（快ｎ（ＥＤＬ－Ｍｎ）群ＣＧＲＰ配布式样不同,即ＳＯＬ－Ｍｎ群含较高比率（ＣＧＲＰ－ＬＩ）阴性和弱阳性Ｍｎ,ＥＤＬ－Ｍｎ群含较高比率ＣＧＲＰ－ＬＩ强阳性Ｍｎ,于

Effect of 5,7-dihydroxytryptamine on the distribution pattern of calcitonin gene-related peptide in different motoneuron pools

The effect of 5,7-dihydroxytryptamine(5,7-DHT)on distribution pattern of calcitonin gene-related peptide(CGRP) in different motoneuron pools was studied in rats. Methods Cholera toxin B subunit coupled with colloidal gold (CB-Au) retrograde labeling combined with CGRP immunocytochemistry technique was mainly used. Results In control group,there was a different distribution pattern of CGRP-LI content in soleus motoneuron (SOL-Mn)and extensor digitorum longus motoneuron (EDL-Mn)pools.SOL-Mn pools had a higher ratio of neurons lacking CGRP-LI/weak CGRP-LI and a lower proportion of strongly CGRP-LI labeled ones.Comparing with control,there was a marked shift to increasing of CGRP-LI content in SOL-Mn pool and slight decrease in EDL-Mn pools 14 days after 5,7-DHT.All the SOL-Mns were CGRP-LI positive and the ratio of CGRP-LI strongly positive Mns significantly increased (P<0.001)while 5,7-DHT did not increase the ratio of CGRP-LI strongly positive Mn in EDL-Mn pool. Conclusions Except muscular motor activity,the different distribution pattern of CGRP-LI content in SOL-Mn and EDL-Mn pools is related to different 5-HT inputs.SOL-Mns may receive more 5-HT inputs.