广西汉族妊娠期糖尿病与HLA-DQA1基因相关性研究

目的探讨广西地区汉族妊娠期糖尿病（GDM）与HIA-DQA1等位基因的相关性。方法采用聚合酶链反应-序列特异性引物法检测50例GDM孕妇和50例正常孕妇的HLA．DQAI基因型。结果GDM孕妇与正常孕妇相比较，HLA-DQA1＊0501基因频率明显升高，差异有显著性（P=0．006）。HLA—DQA1＊0101、0104、0201、0601等位基因频率在GDM孕妇中有增高趋势，但差异没有显著性（P均〉0．05）。GDM孕妇中HLA—DQA1＊0102、0103、0301、0302和0401基因频率有降低趋势，差异亦无著性（P均〉0．05）。结论广西地区汉族GDM与HLA—DQA1基因相关，HLA—DQA1％0501基因可能为广西地区汉族GDM的易感基因，未发现与HIA—DQA1相关的GDM保护基因。

Study on association of HLA-DQA1 gene polymorphism with gestational diabetes mellitus in Guangxi Han nationality

~ Objective To investigate the relationship between HLA-DQA1 gene polymorphism and gestational diabetes mellitus （GDM） in Guangxi Han nationality. Methods Fifty cases of GDM and fifty cases of normal pregnant women are detect HLA-DQA1 alleles by polymerase chain reaction-sequence specific primers （PCR-SSP）. Results The gene frequency of HLA-DQA1 ＊ 0501 increased in GDM group compared with control group （P = 0. 006）. The gene frequencies of HLA-DQA1 ＊ 0101,0lOd ,0201 and 0601 were increased in GDM group,but have no significant difference. The gene frequencies of HLA- DQA1 ＊ 0102,0103,0301,0302 and 0401 were decreased in GDM group, but also have no significant difference. Conclusions HLA-DQA1 gene polymorphism is associated with GDM in Guangxi Han nationality. Alleles HLA-DQA1 ＊ 0501 may be the susceptible gene of GDM in Guangxi Han nationality,but the protective gene of GDM is still not found.