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| 改善葛根素肠道吸收的体外研究 | |
| 引用本文： | 李林,张琼,杨志建,陆阳. 改善葛根素肠道吸收的体外研究[J]. 中草药, 2011, 42(11): 2265-2269 |
| 作者姓名： | 李林 张琼 杨志建 陆阳 |
| 作者单位： | 1. 上海交通大学医学院药学系,上海200025;联合利华上海研究所,上海200335 2. 华东理工大学生物工程学院,上海,200237 3. 联合利华上海研究所,上海,200335 4. 上海交通大学医学院药学系,上海,200025 |
| 摘 要： | 目的研究葛根素肠道吸收的机制,探讨提高其肠道吸收的方法。方法改变实验时间、温度及药物质量浓度,考察葛根素在Caco-2细胞中的转运特性,并考察不同的吸收促进剂对其跨膜转运的影响。结果葛根素的膜渗透性低,被动扩散是其跨膜转运的主要机制;十二烷基磺酸钠(SDS)破坏Caco-2单细胞层的完整性,提高葛根素转运;聚氧乙烯月桂基醚(Brij 35)、牛血清白蛋白(BSA)和分离乳清蛋白对葛根素转运有促进作用,但不影响Caco-2细胞的跨膜电阻(TEER);相对分子质量较高的壳聚糖通过可逆性地降低TEER,促进细胞间转运,提高葛根素的膜渗透性。结论葛根素的膜渗透性低是口服生物利用度低的主要原因,不同吸收促进剂可通过不同机制改善其膜渗透性。 |
| 关 键 词： | 葛根素 肠道吸收 生物利用度 Caco-2细胞 膜渗透性 |
| 收稿时间： | 2011-05-26 |
In vitro investigation on improvement in puerarin intestinal absorption |
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| LI Lin,ZHANG Qiong,YANG Zhi-jian and LU Yang. In vitro investigation on improvement in puerarin intestinal absorption[J]. Chinese Traditional and Herbal Drugs, 2011, 42(11): 2265-2269 | |
| Authors: | LI Lin ZHANG Qiong YANG Zhi-jian LU Yang |
| Affiliation: | LI Lin1,2,ZHANG Qiong3,YANG Zhi-jian2,LU Yang1 1.Department of Pharmacy,School of Medicine,Shanghai Jiao Tong University,Shanghai 200025,China 2.Unilever R&D Shanghai Institute,Shanghai 200335,China 3.College of Bioengineering,East China University of Science and Technology,Shanghai 200237,China |
| Abstract: | Objective To study the mechanism of puerarin(PE) intestinal absorption and investigate the possible methods to improve its intestinal absorption.Methods The characteristics of PE transport across Caco-2 cell monolayer were determined by changing incubation time,temperature,and PE concentration.The effect of different absorption enhancers on its membrane transport permeability was studied as well.Results The intestinal permeability of PE was low and the passive diffusion was the main mechanism for its transp... |
| Keywords: | puerarin(PE) intestinal absorption bioavailability Caco-2 cells membrane permeability |
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