大鼠穴区注射干细胞因子抗体对肥大细胞数量和脱颗粒的影响

目的:观察穴位干细胞因子(SCF)抗体注射及电针后大鼠穴区组织内肥大细胞(MCs)分布及功能变化,探讨电针过程中SCF对大鼠穴区MCs活性的影响。方法:将30只Wistar大鼠随机分为正常组、电针组、注射抗体+电针组,每组10只。电针组选取左侧"胃俞"足三里"穴给予电针,强度0.1 mA,频率2 Hz/15 Hz,针刺25 min。注射抗体+电针组先给予左侧"胃俞"足三里"穴1∶200 SCF抗体稀释液0.1 mL封闭,然后进行电针刺激25 min,其它参数同电针组。乙酰胆碱酯酶组化染色并甲苯胺兰复染法观察各穴区组织内MCs的分布,比较各组"胃俞"足三里"穴区内MCs总量和脱颗粒的差异。结果:与正常组比较,电针组"胃俞"穴区、"足三里"穴区组织MCs总数均升高,注射抗体+电针组两穴区MCs总数均下降,注射抗体+电针组"胃俞"足三里"穴MCs总数与电针组、正常组的差异有统计学意义(均P<0.05)。与正常组比较,电针组、注射抗体+电针组"胃俞"足三里"穴MCs脱颗粒率均显著升高(均P<0.05),电针组和注射抗体+电针组两穴区MCs脱颗粒率的差异无统计学意义(均P>0.05)。结论:SCF抗体穴区注射及电针后,穴区MCs显著减少,SCF是电针过程中促进MCs向穴区迁移、募集的重要因子。

Effect of acupoint-injection of stem cell factor antibody on electroacupuncture induced changes of count and degranulation of subcutaneous mast cells in the local region in rats

Objective To observe the alternations of local mast cells(MCs) in the number and functional activities after electroacupuncture(EA) and injection of stem cell factor(SCF) antibody into "Weishu"(BL 21) and "Zusanli"(ST 36) in rats.Methods Thirty Wistar rats were randomly assigned to normal,EA,and EA+SCF antibody injection(EA-ABI) groups(n=10).EA was applied to the left "Weishu"(BL 21) and "Zusanli"(ST 36) for 25 min.For rats of EA+ABI group,SCF-AB(1∶200,0.1 mL) was respectively injected into BL 21 and ST 36,following by EA.The tissues around the two acupoints areas were sampled to be stained with acetylcholine esterase(AchE) and toluidine blue(TB),respectively.The counts and degranulation ratio of MCs in the acupoint region were recorded and observed under microscope.Results In comparison with the normal group,MCs count was increased significantly in the EA of "Weishu"(BL 21) group(P<0.05),mildly in the EA of "Zusanli"(ST 36) group(P>0.05),and downregulated considerably in both EA-ABI-BL 21 and EA-ABI-ST 36 groups(P<0.05).Compared with the normal group,the degranulation ratio of MCs was increased significantly in EA-BL 21,EA-ST 36,EA-ABI-BL 21 and EA-ABI-ST 36 groups(P<0.05).No significant differences were found between EA-BL 21 and EA-ABI-BL 21 groups,and between EA-ST 36 and EA-ABI-ST 36 groups in the degranulation ratios(P>0.05).Conclusion Local injection of SCF antibody in BL 21 and ST 36 can significantly suppress EA-induced increase of the number and degranulation ratio of local subcutaneous MCs in rats,suggesting that SCF is an important factor affecting the activity and recruitment of MCs around the acupoint during EA.