急性胆管炎时TNF对肝脏的影响

为了研究急性胆管炎时肿瘤坏死因子(TNF)对肝脏的影响及抗TNF单克隆抗体(TNF—McAb)和头孢噻甲羧肟对肝脏的保护作用,本实验在大鼠急性胆管炎模型上,检测血浆TNF、内毒素(ET)水平及血清谷丙转氨酶(ALT)活性,观察肝组织一般结构和超微结构,结果显示:急性胆管炎组大鼠血浆TNF、ET水平及ALT活性显著升高(P<0.01),相应肝脏病理变化显著,TNF—McAb和头孢噻甲羧肟保护组血浆TNF、ET水平及血清ALT活性显著下降,肝组织病理变化较轻,结果提示;TNF是急性胆管炎时肝脏损害的重要介质;TNF—McAb和头抱噻甲羧肟对急性胆道感染肝损害有保护作用。

The influence of TNF to liver in acute cholangi tis

In order to determine,the influence of tumor necrosis factor(TNF)to liver in-jury in acute colangitis and protective effects of TNF -McAb and ceftazidime. 72SD rats were randomizedly divided into four groups,sham-operation group .acute cholangitis group, arid two therapeutic group using TNF-M,cAb and Ceftazidime (in each group n = 8). Acute cholangitis model was made by injecting E. eoli to the common bile duct after ligating it. plasma TNF,ET level and sefum ALT activty of rats were deter-mined in each groups,and microscopic structures and ultrastructures of liver were observed.The results showed TNF. ET lever and serum ALT actvity of acute cholangitis grouprats were significantly higher than that of sham-opertion: group rats (P<0. 01). the pathological changes of livers in acute cholangitis group were prominant. Treated with TNF -McAb and ceftazidime the plasma TNF. ET level and serum ALT activity of acute cholangitis rats decreased significantly(p<0. 05 or P<0. 01),the, pathological changes of livers were milder. These finding indicated that TNF is one of the most important mediatox for liver pathological injury ,and TNF-McAb and ceftazidime take a protective effect for liver injury in a-cute cholangitis rats.