Marimastat对裸鼠原位种植人胃癌生长和转移抑制的实验研究

目的 探讨基质金属蛋白酶抑制剂Marimastat对胃癌生长和转移的抑制作用。方法 采用人胃腺癌细胞株SGC—7901完整组织块棵鼠原位种植，建立胃癌转移模型。移植7天后开始腹腔内注射Marimastat，隔天1次，剂量为0mg/kg(对照组)、10mg/kg、30mg/kg、60mg/kg(治疗组)，共用8周，第9周处死动物，测量原位肿瘤大小、抑瘤率、肿瘤微血管密度(MVD)、MMP—9的高表达率、观察肿瘤转移情况。结果 治疗组原位肿瘤瘤重明显较对照组轻，肿瘤肝脏转移、腹膜转移、肿瘤组织微血管密度和肿瘤组织MMP—9高表达率与对照组比较，均有显著性差异(P＜0．05)，且与Marimastat剂量呈正相关。结论 Marimastat对体内胃癌的生长及转移均有抑制作用。

Matrix Metalloproteinase Inhibitor Marimastat Inhibits Growth and Metastasis of Human Gastric Cancer in Nude Mice

Objective To evaluate the inhibitory effects of matrix metalloproteinase(MMP) inhibitor marimastat on the growth and metastasis of human gastric cancer in nude mice.Methods Metastatic model simulating human gastric cancer was established by orthotopic implantation of intact human tumor tissue into gastric wall of nude mice.Marimastat was injected into the peritoneal cavity at dose of 0 mg/kg,10 mg/kg,30 mg/kg,60 mg/kg every other day for eight weeks.Nine weeks after implantation,the mice were sacrificed and the tumor weight,inhibition rate,microvessel density(MVD),over expression of MMP 9,the presence of metastasis were evaluated respectively.Results Compared with the control group,the tumor weight was significantly lighter in treatment group.The microvessel density was decreased significantly in the treasted mice.Over expression of MM 9,tumor metastasis to the liver and peritoneaum were also significantly inhibited in a dose dependent manner(P<0.05).Conclusion Marimastat has strong inhibitory effect on growth and metastasis of human gastric cancer in nude mice.