cDNA cloning of Entamoeba histolytica histone H3. |
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| Authors: | M F?dinger S Ortner B Plaimauer G Wiedermann O Scheiner M Duchêne |
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| Institution: | Institute of Specific Prophylaxis and Tropical Medicine, University of Vienna, Austria. |
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| Abstract: | E. histolytica has an unusual nuclear structure. Although nucleosome-like particles were observed, no information about histones was available so far. In this paper we describe a cDNA clone with significant homology to H3 histones that was isolated from a lambda Zap library of pathogenic E. histolytica. The complete cDNA encodes a 15 kDa polypeptide, which is shorter by one residue than the human homologue. The amino acid sequence has only 69% identity with human H3.3 histone. Our results indicate that this divergence may contribute to the unusual chromatin structure of E. histolytica. |
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