| 制备抗人血清白蛋白抗体的简单高效方法 |
| |
| 引用本文： | 兰凤华,赵雨田. 制备抗人血清白蛋白抗体的简单高效方法[J]. 南方医科大学学报, 1990, 0(1) |
| |
| 作者姓名： | 兰凤华 赵雨田 |
| |
| 作者单位： | 南京军区福州总医院实验科(兰凤华)，第一军医大学疟疾免疫研究室(赵雨田) |
| |
| 摘 要： | 以简单的酸乙醇法纯化人血清白蛋白(HSA)作为抗原,采用一个高效、经济、快速的免疫方案免疫家兔,得到高效价的抗血清。最后利用ProteinA-Sepharose 4B亲和层析法纯化IgG抗体。
|
| 关 键 词： | 人血清白蛋白 动物免疫 抗血清 IgG 亲和层析 |
Preparation of rabbit anti-human albumin serum and purification of the IgG antibodies |
| |
| Lan Fenghua et al. Preparation of rabbit anti-human albumin serum and purification of the IgG antibodies[J]. Journal of Southern Medical University, 1990, 0(1) |
| |
| Authors: | Lan Fenghua et al |
| |
| Affiliation: | Lan Fenghua et alDepartment of Malaria Immunology |
| |
| Abstract: | We applied a very simple method, TCA-alchol method, to purify human serum albumin. The product is pure albumin by SDS-PAGE. Emulsified with Fre-und's complete adjuvant ( FCA ) , it was used to immunize rabbits by a procedure which lasts only five weeks. Specific anti-serum with high titration ( 1:128) was obtained, as judged by immunoelectrophoresis and counter-immunoelectrophoresis ( CIE ) .Lastly, IgG antibodies in the anti-serum were purified by affinity chro-matography on a Protein-A-Sepharose column. SDS-PAGE and CIE were used in characterizing their purity activity. |
| |
| Keywords: | serum albumin immunization affinity chromatography IgG anti-serum |
| 本文献已被 CNKI 等数据库收录！ |
