| 从土壤中分离的棘阿米巴属CJY/S1和CJY/S2株的18S rDNA基因型鉴定 |
| |
| 引用本文： | 郑善子,玄英花,王月华,申成华,崔春权. 从土壤中分离的棘阿米巴属CJY/S1和CJY/S2株的18S rDNA基因型鉴定[J]. 中国寄生虫学与寄生虫病杂志, 2006, 24(5): 391-392 |
| |
| 作者姓名： | 郑善子 玄英花 王月华 申成华 崔春权 |
| |
| 作者单位： | 1. 延边大学基础医学院免疫学与病原生物学教研部,延吉,133000
2. 吉林医药学院病原生物学教研室,吉林,132013 |
| |
| 基金项目： | 延边大学校科研和教改项目 |
| |
| 摘 要： | 从吉林延边地区土壤中分离的棘阿米巴属CJY/S1和CJY/S2株中提取基因组18S rDNA，PCR扩增、克隆、测序后用分子生物学软件Clustal X进行序列分析，与基因库中已有T1至T12型序列进行比较并构建进化树。结果棘阿米巴土壤分离株Acanthamoeba sp. CJY/S1和CJY/S株的18S rDNA全基因序列分别为2 255 bp和2 252 bp，均属T4基因型。
|
| 关 键 词： | 棘阿米巴 18S rDNA 基因型 分类 |
| 文章编号： | 1000-7423(2006)-05-0391-02 |
| 修稿时间： | 2005-10-25 |
Genetic Identification of Acanthamoeba sp. CJY/S1 and CJY/S2 Isolated from Soil |
| |
| ZHENG Shan-zi,XUAN Ying-hua,WANG Yue-hua,SHEN Cheng-hua,CUI Chun-quan. Genetic Identification of Acanthamoeba sp. CJY/S1 and CJY/S2 Isolated from Soil[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2006, 24(5): 391-392 |
| |
| Authors: | ZHENG Shan-zi XUAN Ying-hua WANG Yue-hua SHEN Cheng-hua CUI Chun-quan |
| |
| Affiliation: | Department of Immunology and Pathogen Biology, Yanbian University, College of Basic Medicine, Yanji 133000, China. |
| |
| Abstract: | Two isolates of Acanthamoeba sp.CJY/S1 and CJY/S2 were received from soil in Yanji of Jilin Province.Full 18S rDNA gene was amplified using PCR,cloned and sequenced.The results were analyzed by software Clustal X.The full length of CJY/S1 and CJY/S2 is 2255 bp and 2252 bp respectively,both belong to T4 genotype. |
| |
| Keywords: | 18S rDNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国寄生虫学与寄生虫病杂志》浏览原始摘要信息 |
|
点击此处可从《中国寄生虫学与寄生虫病杂志》下载全文 |
