Cu2+、Zn2+胁迫对蛹虫草(Cordyceps militaris) 抗氧化酶活性及脂氧化水平的影响

研究了Cu^2＋、Zn^2＋对蛹虫草菌丝体抗氧化酶活性及脂氧化水平的影响。结果表明：高浓度的Cu^2＋抑制菌丝体的生长和细胞内抗氧化酶的活性；高浓度的Zn^2＋对菌丝体的生长也有抑制作用但抗氧化酶的活性变化不大。脂氧化水平随细胞内过氧化状态的变化而变化。蛹虫草菌丝体对Cu^2＋、Zn^2＋有较高的耐受度。

The Effect of Cu2+ and Zn2+ Stress on Antioxidant Enzyme Activity and Lipid Peroxidation Level of Entomogenous Fungal Species Cordyceps Militaris

The effect of Cu~(2+) and Zn~(2+) stress on antioxidant enzyme activity and membrane lipid peroxidation level(LPO) of entomogenous fungal species cordyceps militaris was studied.Cu,and Zn containing superoxide dismutase(Cu,Zn-SOD) and catalase(CAT) activity were detectable in the culture filtrates,and intracellular Cu,Zn-SOD and CAT activity as a proportion protein was the highest in early log phase culture.LPO was estimated based on thiobarbituric acid(TBA) reactivity.The results showed that Cu~(2+) excess could restrain culture growth and antioxidant enzyme activity;Zn~(2+) excess also could restrain culture growth,but antioxidant enzyme activity in the cells appeared to be insensitive to Zn~(2+) excess.The LPO levels in the cells were relative to the cell oxidative conditions.The strain C.militaris has high resistance to Cu~(2+) and Zn~(2+),and has high levels of antioxidant enzyme activities.