脑胶质瘤CT灌注成像与VEGF表达的对照研究

目的运用多层螺旋CT(MSCT)灌注成像的方法获取脑胶质瘤组织相对脑血流容积值(rCBV),探讨rCBV值与肿瘤组织的血管内皮生长因子(VEGF)表达水平的相关性。方法24例脑胶质瘤,低级别胶质瘤8例,间变性胶质瘤10例,胶质母细胞瘤6例。常规CT扫描后,行CT灌注成像,构建分析脑血容量CBV图像,计算并记录肿瘤与正常脑组织的相对脑血流容积rCBV值,免疫组织化学技术测量肿瘤组织的VEGF表达水平,观察CT灌注成像结果与病理分级和VEGF表达水平的相关性。结果低级别胶质瘤组、间变性胶质瘤组及胶质母细胞瘤组的rCBV值分别为3.435±0.4628,6.672±0.8462,9.849±1.2636;VEGF在低级别胶质瘤组、间变性胶质瘤组及胶质母细胞瘤组中表达的ALD值分别为0.3177±0.0768、0.4693±0.0956、0.6368±0.1072,各组胶质瘤的rCBV值均有显著统计学差异(P<0.001),rCBV和VEGF的表达水平密切相关(r=0.86,P<0.001)。结论脑胶质瘤的rCBV和VEGF的表达水平有良好的相关性,多层螺旋CT灌注成像rCBV值可以间接反映脑胶质瘤血管生成的情况。

Correlation of CT perfusion imaging with VEGF expression in gliomas

Objective To obtain relative cerebral blood volume (rCBV) value by using multi-slice helical CT perfusion imaging, to evaluate the correlation between rCBV value and vascular endothelial growth factor (VEGF) expression in human gliomas. Methods Multi-slice helical CT perfusion imaging was performed after routine scanning in 24 patients with brain glioma, including 8 cases of low-grade glioma, 10 cases of anaplastic astrocytoma and 6 cases of glioblastoma. The rCBV values were calculated and the VEGF expressions in tumors were determined by immunohistochemistry. The correlation of CT perfusion imaging with the pathologic grade and VEGF expression was analyzed. Results The rCBV value in low-grade glioma, anaplastic astrocytoma and glioblastoma were 3.435±0.4628, 6.672±0.8462, 9.849±1.2636, respectively; and the ALD values of VEGF expression in different grade glioma groups were 0.3177±0.0768, 0.4693±0.0956, 0.6368±0.1072 respectively. Statistically significant difference in rCBV existed between different groups. The rCBV value was closely correlated with VEGF expression in glioma. Conclusion The VEGF expression of glioma has a positive correlation with rCBV value in CT perfusion imaging. CT perfusion imaging can indirectly reflect the angiogenesis activity of brain glioma.