普罗布考对受损内皮细胞增殖和功能修复的促进作用

目的 :研究普罗布考对受损内皮细胞增殖和再生内皮细胞功能修复的影响。方法 :分别采用MTT比色法和PCNA免疫组化法测定体外培养的人脐静脉内皮细胞所处增殖状态 ;以兔胸主动脉血管环对乙酰胆碱舒张反应和内皮细胞分泌NO、PGI2 的能力评价内皮细胞功能状况。结果 :LPC作用 2 4h可导致内皮细胞增殖显著受抑及分泌NO、PGI2 功能受损 ,不同剂量普罗布考 (2 0 ,4 0 ,80 μmol·L- 1)可促进LPC损伤的内皮细胞增殖 (5 5 .5 % ,5 9.3% ,72 .2 %vs 31.7% ,P <0 .0 1) ,同时提高血管成型术后兔胸主动脉血管环内皮依赖性舒张功能 (6 6 .6 3%vs 2 5 .95 % ,P <0 .0 1) ,并修复LPC损伤的内皮细胞分泌NO、PGI2 的功能 (P <0 .0 1)。结论 :普罗布考可促进受损内皮细胞增殖和再生内皮细胞功能修复。

Effects of probucol on proliferation and function recovery of injured endothelial cells

AIM: To study the effects of probucol on injured endothelial cells and regenerated endothelium. METHODS: In cultured human umbilical vein endothelial cells (huVECs), various concentration of probucol (20, 40, 80 μmol*L-1) were given 30 min after lysophophatidylcholine (LPC, 20 mg*L-1) treatment. MTT assay and PCNA immunohistochemical staining assay were used to evaluate the proliferation of injured huVECs. The function of endothelium was estimated by measuring the relaxation response of PTA rabbits thoracic aorta rings to acetylcholine and by measuring NO and PGI2 release of cultured huVECs. RESULTS: LPC treatment for 24 h obviously inhibited huVECs proliferation and attenuated huVECs release of NO and PGI2 (P< 0.01). Probucol (20, 40, and 80 μmol*L-1) promoted the proliferation of injured huVECs with the growth rate from 31.7% to 55.5%, 59.3% and 72.2% respectively as shown by MTT assay. Probucol also enhanced vascular relaxation response of rabbits thoracic aorta rings to acetylcholine from 25.95% to 66.63% and increased NO and PGI2 release of injured huVECs. CONCLUSION: Probucol can promote proliferation and function recovery of impaired endothelial cells.