NO供体亚硝基化合物-18对大鼠成骨细胞的影响

目的 探讨NO供体亚硝基化合物-18(NOC-18)对大鼠成骨细胞增殖和分化、碱性磷酸酶(ALP)活性和Ⅰ型胶原蛋白表达的影响。方法 采用酶消化法和骨组织块法联合分离培养新生SD大鼠颅骨成骨细胞，然后将不同浓度的NOC-18作用于大鼠成骨细胞，采用噻唑蓝(MTY)比色法检测细胞增殖能力，测定细胞ALP活性。免疫细胞化学染色检测Ⅰ型胶原蛋白的表达，观察NOC-18对大鼠成骨细胞增殖和分化能力的影响。结果 NOC-18可显著促进大鼠成骨细胞增殖，提高成骨细胞ALP活性和Ⅰ型胶原蛋白的表达。结论 适量浓度的NOC-18可通过刺激成骨细胞的增殖和分化，促进骨形成，为预防和治疗骨质疏松提供了理论和实验依据。

Effect of NO donor, nitrosocompound-18 on proliferation and differentiation of rat osteoblasts

Objective To investigate the effect of an NO donor, nitrosocompound-18(NOC-18) on the proliferation and differentiation of rat osteoblasts in vitro. Methods Osteoblasts were isolated from the calvariae of new-born SD rats by combined means of enzymatic digestion and bone tissue block, and cultured with alpha-modified MEM. Different concentrations of NOC-18 were added to rat osteoblasts and incubated for 72 hours. Cell proliferation was measured by the tetrazolium salt test (MTT), as well as the activity of alkaline phosphatase (ALP) was assayed. The expression of type I collagen was analyzed with immunohistochemical stain. Results NOC-18 increased the osteoblastic proliferation significantly and enhanced alkaline phosphatase (ALP) activity and the expression of type I collagen in rat osteoblasts. Conclusions The fitting concentration of NOC-18 can promote bone formation by stimulating the proliferation and differentiation of osteoblasts. And this could become a new preventive and therapeutic avenue for osteoporosis.