LYVE-1蛋白在小鼠碱烧伤角膜的表达及意义

目的观察淋巴管内皮透明质酸受体(LYVE-1)在小鼠碱烧伤角膜内的表达情况,探讨角膜新生淋巴管形成的时间过程及角膜疾病后新生淋巴管形成的作用。方法应用NaOH溶液制作小鼠角膜碱烧伤模型,分别于角膜碱烧伤后第1d、3d、5d、7d和12d取材。采用免疫组化法,观察正常角膜和碱烧伤后不同时间段角膜内LYVE-1的表达情况。结果在正常角膜组织中,LYVE-1表达于角膜上皮细胞和内皮细胞内。在角膜碱烧伤后1d、3d和5d,LYVE-1主要表达于角膜上皮内及入侵角膜基质的炎性细胞内;碱烧伤后7d,可见大量LYVE-1呈条索样表达于角膜基质中,并可见少量LYVE-1阳性表达于开放状态的淋巴管;碱烧伤后12d,角膜基质内新生淋巴管数量增多。结论正常角膜组织储备LYVE-1生物因子,在炎性角膜中,LYVE-1可能在角膜新生淋巴管运输透明质酸(HA)的过程中发挥重要作用。

Expression and significance of LYVE-1 in mouse cornea after alkali burning

Objective To investigate the expression of lymphatic vessel endothelial hyaluronic acid receptor（LYVE-1）in mouse cornea to explore the mechanism of corneal lymphangiogenesis after alkali burns.Methods The corneal alkali burning models were built by sodium hydroxide,corneas were excised at the stages of 1d,3d,5d,7d and 12d after alkali burning respectively.The expression of LYVE-1 was observed by immunohistochemical stain.Results The positive staining of LYVE-1 protein was found in epithelium and endothelium of normal cornea.The positive expression of LYVE-1 was detected in epithelium and infiltrated inflammatory cells in cornea stroma at 1d,3d and 5d after corneal alkali burning,a lot of strip-like LYVE-1 stainig and very few newly formed lymphatic vessels were seen at 7d after corneal alkali burning,many neolymphatic vessels in corneal stroma showed LYVE-1 staining at 12d after corneal alkali burning.Conclusion LYVE-1 may play an important role in transporting hyaluronan（HA） in inflammatory cornea.