2-脱氧葡萄糖诱导的内质网应激预处理对大鼠脑缺血再灌注损伤的保护作用

目的研究2-脱氧葡萄糖(2-DG)诱导内质网应激(ERS)预处理对大鼠脑缺血再灌注的保护作用。方法 64只雄性SD大鼠随机均分为假手术组(SH组)、缺血/再灌注组(I/R组)、2-DG诱导的ERS预处理组(IP组)、IP+I/R组。采用TUNEL法检测CA1区凋亡细胞,免疫组化法、Western-Blot法检测p-JNK蛋白在海马CA1区的表达变化。结果与对照组比较,I/R组海马CA1区锥体神经元排列紊乱及变性坏死,形态正常椎体细胞百分数减少,凋亡细胞数目明显增加,脑组织p-JNK表达水平增加;与I/R组相比,IP+I/R组形态正常锥体细胞明显增加,凋亡细胞数目明显减少,脑组织表达较I/R组明显减少。结论 2-DG诱导的ERS对脑缺血再灌注损伤有保护作用,可能与其抑制细胞凋亡和p-JNK表达相关。

Protective effect of endoplasmic reticulum stress pretreatment induced by 2-deaeration glucose on brain in ischemia/reperfusion rats

Objective To study the protection and underlying mechanism of endoplasmic reticulum stress（ERS） pretreatment induced by 2-deaeration glucose （2-DG） on hippocampal CA1 region neurons in ischemidreperfusion rats. Methods A total of 64 SD rats were randomly divided into： sham group（SH）, ischemia/reperfusion group（I/R）, endoplasmic reticulum stress preconditioning group（IP） and IP＋I/R group. TUNEL was used to test cell apoptosis, immunohistochemistry and Western-Blot were used to detect the levels of p-JNK expression. Results The dearrangement and degeneration and necrosis pyramidal neurons were seen in hippocampal CA1 region in I/R group, the number of survival pyramidal cells was decreased, the number of apoptotic cells and p-JNK protein expression level increased significantly in hippocampal CA1 region in I/R group than in control group. The number of survival pyramidal （：ells was increased, the number of apoptotic cells and p-JNK protein expression level decreased significantly in hippocampal CA1 region in IP＋I/R group than in I/R group. Conclusions The protective effect of ERS pretreatment induced by 2-DG on hippocampal CAI neurons of ischemia/reperfusion rat might be related to the inhibition of apoptotic cells and p-JNK protein expression.