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| Wee1基因在大鼠生后发育不同阶段与压力负荷后心肌组织的差异表达 | |
| 引用本文： | 张凌志,邸菁,徐峰,柏树令,高杰,佟浩.Wee1基因在大鼠生后发育不同阶段与压力负荷后心肌组织的差异表达[J].解剖科学进展,2012,18(3):227-229,234. |
| 作者姓名： | 张凌志 邸菁 徐峰 柏树令 高杰 佟浩 |
| 作者单位： | 1. 中国医科大学附属盛京医院急诊科,辽宁沈阳,110001 2. 中国医科大学基础医学院人体解剖学教研室,辽宁沈阳,110001 3. 中国医科大学附属一院心内科,辽宁沈阳,110001 |
| 基金项目： | 辽宁省教育厅科研计划项目，辽宁省自然科学基金，辽宁省教育厅课题 |
| 摘 要： | 目的检测wee1基因在大鼠生后不同发育阶段和压力负荷性心肌肥大心肌组织中的表达情况,探讨心肌细胞增殖调控的相关机制。方法采用升主动脉缩窄方法制作压力负荷性心肌肥大动物模型,采用RT-PCR技术检测大鼠生后不同发育阶段和肥大心肌组织wee1基因的mRNA表达。结果 wee1基因在大鼠生后0天呈低表达,生后第3天高表达(P<0.01),之后至成年呈低水平表达;与假手术组相比,压力负荷性肥大心肌组织wee1基因表达水平明显上调(P<0.01)。结论 wee1基因在大鼠生后发育过程中和在压力负荷性心肌肥大组织呈差异表达。 |
| 关 键 词： | Wee1 心肌细胞 心肌肥大 RT-PCR 大鼠 |
Discordant expression of Wee 1 gene in hearts of postnatal developmental process and pressure overload hypertrophy of rat |
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| ZHANG Ling-zhi , DI Jing , XU Feng , BAI Shu-ling , GAO Jie , TONG Hao.Discordant expression of Wee 1 gene in hearts of postnatal developmental process and pressure overload hypertrophy of rat[J].Progress of Anatomical Sciences,2012,18(3):227-229,234. | |
| Authors: | ZHANG Ling-zhi DI Jing XU Feng BAI Shu-ling GAO Jie TONG Hao |
| Institution: | 1.Department of Emergency,Shengjing Hospital,2.Department of Anatomy,3.Department of Cardiology,First Hospital Affiliated,China Medical University,Shenyang 110001 China) |
| Abstract: | Objective To study the expression of wee 1 gene in hearts of postnatal developmental process and pressure overload hypertrophy of rat.Methods By stenosis of the ascending aortic artery,pressure overload hypertrophy rats（n=10） were conducted,sham operated rats（n=10）were selected randomly as non stenosis control,wee 1 mRNA expression was detected by RT-PCR method.Results The expression level of wee 1 mRNA in rat hearts was lower at postnatal 0 day,reached peak at postnatal 3 days（0.01）,and continued lower expression till adult.However,significant higher expression level was detected in hypertrophy myocardium of rat compared to sham（0.01）.Conclusion The wee 1 gene showed discordant expression in myocardium of the postnatal development and pressure overload hypertrophy rat. |
| Keywords: | wee 1 gene cardiomyocyte hypertrophy RT-PCR rat |
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