| 重组人促红细胞生成素对脑缺血再灌注小鼠海马水通道蛋白-1表达的作用 |
| |
| 引用本文： | 朱小兵,张云飞,李洪鹏. 重组人促红细胞生成素对脑缺血再灌注小鼠海马水通道蛋白-1表达的作用[J]. 解剖科学进展, 2012, 18(3): 216-218 |
| |
| 作者姓名： | 朱小兵 张云飞 李洪鹏 |
| |
| 作者单位： | 1. 中国医科大学基础医学院 人体解剖学教研室 沈阳110001
2. 中国医科大学附属第一医院超声科 沈阳110001 |
| |
| 基金项目： | 国家自然科学基金资助项目 |
| |
| 摘 要： | 目的探讨重组人促红细胞生成素(recombinant human erythropoietin,rHuEPO)对脑缺血再灌注小鼠水通道蛋白-1(aquaporin1,AQP1)表达的作用。方法 96只昆明小鼠随机分为假手术组(Sham)、rHuEPO治疗组、缺血再灌注组(I/R),每组32只,依据缺血后再灌注不同时间点再细分6h、24h、3d、7d四个小组。利用免疫组织化学方法检测各组小鼠海马组织AQP1的表达;利用Westernblot方法检测各组小鼠海马AQP1的表达。结果免疫组化和Westernblot结果发现,缺血再灌注组AQP1蛋白表达量明显高于假手术组(P<0.05),而rHuEPO治疗组AQP1蛋白表达量则明显少于缺血再灌注组(P<0.05)。结论 rHuEPO能够下调脑缺血再灌注小鼠AQP1的表达。
|
| 关 键 词： | 缺血再灌注 重组人促红细胞生成素 水通道蛋白1 小鼠 |
The effect of recombinant human erythropoietin on the expression of AQP1 in cerebral ischemia-reperfusion mouse |
| |
| ZHU Xiao-bing , ZHANG Yun-fei , LI Hong-peng. The effect of recombinant human erythropoietin on the expression of AQP1 in cerebral ischemia-reperfusion mouse[J]. Progress of Anatomical Sciences, 2012, 18(3): 216-218 |
| |
| Authors: | ZHU Xiao-bing ZHANG Yun-fei LI Hong-peng |
| |
| Affiliation: | 1.Department of Anatomy,College of Basic Medical Sciences;2.Department of Ultrasound,the First Hospital,China Medical University,Shenyang 110001 China) |
| |
| Abstract: | Objective To study the expression of aquaporin1（AQP1） in cerebral ischemia-reperfusion mice after recombinant human erythropoietin（rHuEPO） was injected.Methods 96 Kunming mice were randomly divided into the sham group,ischemia-reperfusion group,recombinant human erythropoietin group at different time points（6h,24h,3d and 7d） on average.The expression of AQP1 in hippocampus was examined by immunochemistry and western blot methods.Result The results of immunochemistry and western blot showed that the expression level of AQP1 increased significantly in I/R group than in sham group（ 0.05）,but decreased significantly in recombinant human erythropoietin group compared with I/R group（ 0.05）.Conclusion recombinant human erythropoietin could downregulate the expression level of AQP1 in cerebral ischemia-reperfusion mice |
| |
| Keywords: | cerebral ischemia-reperfusion recombinant human erythropoietin aquaporins 1 mouse |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
