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| 基质金属蛋白酶—9反义寡核苷酸对前单核细胞THP—1粘附及基质金属蛋白酶—9表达的实验研究 | |
| 引用本文： | 冷启新,李瑞祥,刘执玉.基质金属蛋白酶—9反义寡核苷酸对前单核细胞THP—1粘附及基质金属蛋白酶—9表达的实验研究[J].解剖学杂志,2001,24(3):265-268. |
| 作者姓名： | 冷启新 李瑞祥 刘执玉 |
| 作者单位： | 1. 潍坊医学院解剖学教研室,潍坊,261041 2. 华西医科大学解剖学教研室 3. 山东医科大学解剖学教研室 |
| 摘 要： | 目的：探讨基质金属蛋白酶－9（Matrix Metalloproteinase-9,MMP-9)在前单核细胞THP－1分化过程中的作用。材料和方法：采用硫化修饰的正，反义寡核苷酸，通过基因转染，观测其对THP－1粘附及用流式细胞仪检测MMP－9的表达。结果：MMP－9反义寡核苷酸可有效的抑制THP－1的粘附和MMP－9的表达。结论：MMP－9在前单核细胞的分化过程中起重要的作用。 |
| 关 键 词： | 细胞分化 基质金属蛋白酶－9 基因转染 前单核细胞 实验研究 白血病 |
EXPERIMENTAL RESEARCH OF MATRIX METALLOPRO- TEINASE-9 ANTISENSE OLIGONUCLEOTIDE ON THP-1 ADHESION AND MMP-9 EXPRESSION |
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| Leng Qixin ,Li Ruixiang ,Liu Zhiyu.EXPERIMENTAL RESEARCH OF MATRIX METALLOPRO- TEINASE-9 ANTISENSE OLIGONUCLEOTIDE ON THP-1 ADHESION AND MMP-9 EXPRESSION[J].Chinese Journal of Anatomy,2001,24(3):265-268. | |
| Authors: | Leng Qixin Li Ruixiang Liu Zhiyu |
| Institution: | Leng Qixin 1,Li Ruixiang 2,Liu Zhiyu 3 |
| Abstract: | To study the effect of matrix metalloproteinase-9 in the process of pro-monocyte THP-1 differentiation.Methods:By using phosphorthioated MMP-9 sense and antisense oligonucleotide and gene transfection,MMP-9 expression and the effect of MMP-9 antisense were studied.Results:MMP-9 was highy expressed during THP-1 differentiation and MMP-9 antisense could inhibit MMP-9 expression.Conclusion.MMP-9 is important for THP-1 differentiation. |
| Keywords: | cell cell differentiation matrix metalloproteinasee-9 gene transfection |
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