黄豆苷元对仔猪肠道微生物区系的影响

目的:研究黄豆苷元对仔猪肠道微生物区系的影响。方法:选取江苏某猪场产期相近、胎次相似数目相当的杜/长/大三元杂交仔猪6窝,随机分为2组,每组三窝。一组为对照组,按常规饲养,另一组为处理组,在7、9、11d每头猪强饲大豆黄酮(10mg/L)1ml、2ml、3ml。两组均于21d断奶。于14、21、24、35d每窝各屠宰一头,无菌采集十二指肠、空肠、回肠、盲肠、结肠和直肠食糜。食糜细菌16S rDNA的V6-V8可变区经PCR扩增,扩增产物经变性梯度凝胶电泳(DGGE)电泳后再进行多样性分析,并对特殊条带进一步克隆测序。结果:饲喂大豆黄酮后,处理组仔猪各肠段的DGGE条带数均高于对照组,盲肠、结肠和直肠中细菌的多样性指数增加;与对照组相比,在35d时处理组大肠中出现3条优势条带,经克隆测序后与GenBank登录序列比较,发现它们分别与butyrate-producing bacterium SL7/1,Clostridium butyricum(NCIMB8082),Ruminococcus obeum clone 1-4最相似,相似性分别为95%,95%和96%。结论:黄豆苷元可使仔猪大肠的微生物区系多样性增加,并且可选择性地促进某些菌群的富集。

EFFECT OF DAIDZEIN ON THE INTESTINAL FLORA OF PIGLETS

Objective: To investigate the effect of daidzein on the intestinal microflora of piglets using 16S rDNA-based approaches.Method: Six litters of piglets were allocated in two groups,control group and treatment group,three litters each.The piglets of the treatment group were fed with daidzein orally at D 7,9 and 11.They were all weaned at D 21.One piglet of each group was slaughtered in D 14,21,24 and 35,respectively.The digesta of duodenum,jejunum,ileum,caecum,colon and rectum were collected and used for DNA extraction and followed by PCR/DGGE analysis.The diversity of intestinal microbial populations of the piglets was determined based on the Shannon’s index.The 16S rDNA sequences of intestinal bacterial species were analysed.Results: Piglets of the treatment group showed a higher bacterial diversity and more DGGE bands compared with that of the control group at D 24 and D 35.Three DGGE bands were present only in the large intestine of the treatment group,and their corresponding clones had their 16S rDNA sequences most close to the species of butyrate-producing bacterium SL7/1,Clostridium.butyricum,Ruminococcus obeum,respectively.Conclusion: Daidzein could increase the diversity of the piglets intestional bacterial flora,and stimulate some specific bacteria to grow.