| 脾阴虚证大鼠肝组织的MDA含量端粒长度变化的实验研究 |
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| 引用本文： | 柳承希,王彩霞,崔家鹏.脾阴虚证大鼠肝组织的MDA含量端粒长度变化的实验研究[J].中华中医药学刊,2007,25(1):71-72. |
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| 作者姓名： | 柳承希 王彩霞 崔家鹏 |
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| 作者单位： | 辽宁中医药大学中医基础教研室,辽宁,沈阳,110032 |
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| 摘 要： | 目的：观察脾阴虚证大鼠肝组织的MDA含量、端粒长度变化，探讨脾阴虚证的分子生物学病理机制。方法：采用饮食不节、劳倦过度等复合因素塑造大鼠脾阴虚证模型，用硫代巴比妥酸（TBA）法和Southem—blot法测定脾阴虚证大鼠肝组织的MDA含量、端粒长度变化。结果：与正常对照组比较，脾阴虚证大鼠肝组织的丙二醛（MDA）含量增多、端粒长度显著缩短。结论：在脾阴虚状态下，机体内自由基蓄积损伤端粒，其损伤可能导致肝组织端粒长度缩短。
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| 关 键 词： | 脾阴虚证 自由基 丙二醛(MDA) 端粒 |
| 文章编号： | 1673-7717（2007）01-0071-02 |
| 收稿时间： | 2006-08-15 |
| 修稿时间： | 2006-08-15 |
Experimental Study on the Content of Maleic Dialdehyde and the Length of Telomere in Liver of Rat with Spleen Yin Deficiency Syndrome |
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| SeungHee YU,WANG Cai-xia,CUI Jia-peng.Experimental Study on the Content of Maleic Dialdehyde and the Length of Telomere in Liver of Rat with Spleen Yin Deficiency Syndrome[J].Chinese Archives of Traditional Chinese Medicine,2007,25(1):71-72. |
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| Authors: | SeungHee YU WANG Cai-xia CUI Jia-peng |
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| Institution: | Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China |
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| Abstract: | Objective:To observe the contentof Maleic Dialdehydeand the length of telomere in liver of rat with spleen Yin deficiency syndrome.To discuss the pathomechanismof molecule biology of spleen Yin deficiency syndrome.Methods:The rat of spleen Yin deficiency syndrome was moldedby composite factors of overstrainand intemperance of taking food etc.The methods of Thibabituric acid and Southern blotting were used to determine the contentof Maleic Dialdehydeand the length of telomere.Result: compared with normal control,the contentof Maleic Dialdehydein liver of rat with spleen Yin deficiency syndrome was increased and lenth of telomere was shortened.Conclusion: In the state of spleen Yin deficiency syndrome,the accumulation of free radical may damage the telomere,and that may result in telomere shortening in liver. |
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| Keywords: | spleen yin deficiency syndrome free radical maleie dialdehyde telomere |
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