超抗原TSST-1之靶TCRVβHV4序列的特征分析

首先对人和小鼠的TCRVβ的氨基酸序列进行多序列对准,就Vβ之HV4片段进行比较,分析与超抗原TSST-1结合的四种Vβ（小鼠Vβ3、Vβ15、Vβ17和人Vβ2）之HV4序列内是否存在特定的氨基酸残基排列主型。结果发现:小鼠Vβ3和Vβ17的HV4具有特异的RFSAXCXSNS主型,而小鼠Vβ15和人β2的HV4则含独特的KFXIXH主型。提示:与TSST-1结合的四种Vβ所对应的T细胞识别表位可能不止一个。

Analysis of Specificity in TCRVP HV4 Sequences Targeted by Superantigen TSST-1

The sequences of human and mouse TCR Vβ families were aligned , and the HV4 residues in the Vβ s were compared and analyzed. Whether HV4 sequences of four Vβs (mouse Vβ3, 15, 17 and human Vβ2) targeted by the superantigen TSST-1 contained any specific motif was examined. The results showed that HV4 sequences of mouse Vβ 3 and Vβl7 contained the specific RFSAXCXSNS motif, while those of mouse Vβ 15 and human Vβ2 contained unique KFXIXH motif. The data indicate that there are possibly more than one superantigenic T cell epitopes in TSST-1 molecule.