| 胰腺癌组织中Midkine的表达与细胞增殖及凋亡的关系 |
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| 引用本文： | 马松林,赵秋,王渝,龚勇,杨芳,王颖,覃华. 胰腺癌组织中Midkine的表达与细胞增殖及凋亡的关系[J]. 中国现代医学杂志, 2008, 18(3): 350-353,357 |
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| 作者姓名： | 马松林 赵秋 王渝 龚勇 杨芳 王颖 覃华 |
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| 作者单位： | 1. 华中科技大学同济医学院附属同济医院,消化内科,湖北,武汉,430030;武汉科技大学附属医院,消化内科,湖北,武汉,430064
2. 华中科技大学同济医学院附属同济医院,消化内科,湖北,武汉,430030 |
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| 摘 要： | 目的研究Midkine(MK)蛋白在人胰腺癌组织中的表达与肿瘤细胞增殖和凋亡之间的关系及意义。方法收集人胰腺癌组织标本49例,免疫组化SP法检测MK蛋白和增殖细胞核抗原Ki67的表达,原位末端标记(TUNEL)法检测细胞凋亡指数,另取同期15例正常胰腺组织作对照。结果15例正常胰腺组织中均未发现MK和Ki67阳性表达,细胞凋亡较少(0.63±0.21);77.1%(35/49)的胰腺癌组织中有MK阳性表达,其表达与肿瘤组织学分级、临床分期和淋巴结转移有关(P<0.05);49例胰腺癌组织中Ki67平均标记指数(LI)为(31.67±15.41),平均凋亡指数(AI)为(5.93±4.18);MK表达阳性组Ki67平均标记指数为(35.27±16.03),显著高于阴性组的(22.67±13.66)(P<0.05);MK表达阳性组凋亡指数为(5.13±2.69),显著低于阴性组的(7.93±6.65)(P<0.05)。结论MK蛋白可作为反映胰腺癌生物学行为的指标之一,其在胰腺癌中的表达可能起到促进肿瘤细胞增殖和抑制凋亡的作用,MK基因可能成为胰腺癌治疗的新靶点。
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| 关 键 词： | 胰腺癌 Midkine 细胞增殖 细胞凋亡 胰腺癌治疗 癌组织 Midkine 表达阳性 肿瘤细胞增殖 抑制凋亡 关系 pancreatic cancer apoptosis cell proliferation relationship 新靶点 基因 作用 指标 癌生物学行为 阴性 标记指数 淋巴结转移 临床分期 |
| 文章编号： | 1005-8982（2008）03-0350-04 |
| 收稿时间： | 2007-08-26 |
| 修稿时间： | 2007-08-26 |
Expression of midkine and its relationship with cell proliferation and apoptosis in pancreatic cancer |
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| MA Song-lin,ZHAO Qiu,WANG Yu,GONG Yong,YANG Fang,WANG Ying,QIN Hua. Expression of midkine and its relationship with cell proliferation and apoptosis in pancreatic cancer[J]. China Journal of Modern Medicine, 2008, 18(3): 350-353,357 |
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| Authors: | MA Song-lin ZHAO Qiu WANG Yu GONG Yong YANG Fang WANG Ying QIN Hua |
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| Affiliation: | MA Song-lin1,2,ZHAO Qiu1,WANG Yu1,GONG Yong1,YANG Fang1,WANG Ying1,QIN Hua1 (1.Department of Gastroenterology,Tongji Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan,Hubei 430030,P.R.China,2.Department of Gastroenterology,Affiliated Hospital of Wuhan University of Science , Technology,Hubei 430064,P.R.China) |
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| Abstract: | Objective To investigate the expression and significance of Midkine(MK) protein and its relationship with cell proliferation and apoptosis in human pancreatic cancer. Methods The immunohistochemical staining (SP) method was used to detect the expression of MK protein and proliferating cell nuclear antigen Ki67 in 49 cases of pancreatic cancer tissues. TUNEL method was used to detect apoptotic index. 15 cases of normal pancreatic tissues were selected as controls. Results The expression of Ki67 and MK were n... |
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| Keywords: | pancreatic cancer Midkine proliferation apoptosis |
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