两种方法处理后布尿布中微生物含量和种类的比较研究

目的:比较使用后经医院常规消毒的布尿布和家庭热水清洗处理后的布尿布中的微生物含量和种类的差别。方法:使用后的布尿布经医院常规消毒和家庭热水清洗处理后分别检测了细菌总数、霉菌/酵母菌、大肠杆菌的含量以及铜绿假单胞菌和耐甲氧西林金黄色葡萄球菌。结果:家庭热水清洗处理后的布尿布中64份尿布均检测出细菌,细菌总数的平均值为1450348 cfu/g;对32份样品中霉菌/酵母菌进行了检测,其中30份尿布中检测为阳性,其平均值为726.6 cfu/g;7份尿布中检测出大肠杆菌,平均值为721.1 cfu/g;对铜绿假单胞菌的鉴定均为阴性;46份尿布耐甲氧西林的金黄色葡萄球菌(MRSA)鉴定为阳性;经医院常规消毒后的布尿布中55份尿布中均检测出细菌,细菌总数的平均值为30.9 cfu/g;18份尿布中检测出霉菌/酵母菌,平均值为1.1 cfu/g;64份尿布中均未检出大肠杆菌;对铜绿假单胞菌的鉴定均为阴性;9份尿布耐甲氧西林金黄色葡萄球菌鉴定为阳性。采用经医院常规消毒方法的多重带菌率明显低于家庭热水清洗方法。结论:使用后经过医院常规消毒的布尿布中微生物的浓度明显低于家庭热水清洗处理后的布尿布,且霉菌/酵母菌、耐甲氧西林金黄色葡萄球菌和大肠杆菌是布尿布中的主要微生物。

Comparison of quantity and category of microorganism in cloth diapers disinfected by two different methods

Objective:To compare the quantity and category of microorganism in cloth diapers disinfected by hospital routine disinfection and domestic hot water cleaning.Methods:Cloth diapers were selected from those,which were disinfected by hospital routine disinfection and domestic hot water cleaning respectively.Amount of bacteria,fungus/yeast,Escherichia coli and Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus were detected.Results:Sixty-four cloth diapers disinfected by domestic hot water cleaning were detected with bacteria,the mean value was 1450348 cfu/g;seven diapers were detected with E.coli,the mean value of 721.1 cfu/g;thirty diapers of thirty-two diapers were detected with fungus,the mean value was 726.6 cfu/g;identification of Pseudomonas aeruginosa in all the diapers were negative;identification of methicillin-resistant Staphylococcus aureus(MRSA) were positive in forty-six diapers.In sixty-four cloth diapers disinfected by hospital routine disinfection,fifty-five diapers were detected with bacteria,the mean value was 30.9 cfu/g;none diapers were detected with E.coli,eighteen diapers were detected with fungus/yeast,the mean value was 1.1 cfu/g;identification of Pseudomonas aeruginosa in all the diapers were negative;identification of methicillin-resistant Staphylococcus aureus were positive in nine diapers.The multiple carrier rate of cloth diaper disinfected by hospital routine disinfection was lower than that with domestic starched disinfection of 90 degree water.Conclusion:The concentration of microorganism in cloth diapers disinfected by domestic hot water cleaning was higher than that by hospital routine disinfection,and fungus/yeast,methicillin-resistant Staphylococcus aureus and Escherichia coli were the major pathogens in cloth diapers.